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Updated: May 5, 2026
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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
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Hematopoietic stem cells: do they have a role in keloid pathogenesis?
Ola Ahmed Bakry1, Rehab Munir Samaka, Mohamed Ahmed Basha
1Department of Dermatology , Andrology and STDs .
Ultrastructural Pathology
|December 11, 2013
Summary
Hematopoietic stem cells, marked by CD34 and c-KIT, show increased presence in keloid scars. Targeting these stem cells may offer a new therapeutic approach for keloid treatment.
Area of Science:
- Dermatology
- Oncology
- Stem Cell Biology
Background:
- Keloids are benign fibroblast neoplasms linked to altered cytokine profiles.
- The role of stem cells in keloid formation remains unclear.
- Previous research suggests stem cells contribute to skin tumor development.
Purpose of the Study:
- To investigate the immunoreactivity of CD34 and c-KIT antibodies in keloid lesions.
- To compare stem cell marker expression in keloid tissue versus normal skin.
- To explore the potential role of stem cells in keloid pathogenesis.
Main Methods:
- Immunohistochemical analysis of CD34 and c-KIT in 30 keloid and 30 normal skin biopsies.
- Quantitative assessment of antibody expression intensity and H score.
- Comparison of marker expression between lesional, perilesional, and normal skin.
Main Results:
- CD34 immunoreactivity was found in 76.7% of keloid cases, with upregulated expression in keloid and perilesional tissues compared to normal skin.
- c-KIT expression was positive in all keloid cases, showing significantly higher intensity and H score in keloid and perilesional tissues versus normal skin.
- Basal keratinocytes exhibited increased staining in lesional skin compared to perilesional tissue.
Conclusions:
- Hematopoietic stem cells may play a role in keloid pathogenesis.
- Perilesional accumulation of stem cells warrants further investigation.
- Targeting stem cell populations could be a future therapeutic strategy for keloids.
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