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Tissue tablet method: an efficient tissue banking procedure applicable to both molecular analysis and frozen tissue

Nobuhiro Torata1, Kenoki Ohuchida, Shin Akagawa

  • 1Bachelor of Health Science, Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 811-8582, Japan.

Human Pathology
|December 11, 2013
PubMed
Summary

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A novel tissue tablet method improves frozen tissue sample handling and storage for research. This efficient technique preserves tissue quality for molecular analysis and frozen tissue microarrays, overcoming limitations of traditional methods.

Area of Science:

  • Biotechnology
  • Histology
  • Tissue Banking

Background:

  • Current tissue banking methods, using cryovial tubes or plastic molds with optimal cutting temperature (OCT) compound, are over a decade old and present challenges like sample adhesion and large storage requirements.
  • These limitations hinder efficient utilization of frozen human tissues for critical research applications.

Purpose of the Study:

  • To establish and evaluate an improved tissue freezing and storage procedure, termed the "tissue tablet method."
  • To assess the applicability of this method for both molecular analyses and the construction of frozen tissue microarrays.
  • To compare the quality of tissue processed with the tissue tablet method against conventional OCT blocks.

Main Methods:

  • Tissue samples were fragmented and embedded into tablet-shaped OCT compound using a custom tissue-freezing plate.
Keywords:
CryopreservationFrozen sectionFrozen tissue microarray tissue bankIn situ hybridizationTissue collectionTissue freezing procedure

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  • Tablet-shaped samples were sectioned and stored in cryovial tubes.
  • Frozen tissue microarrays were constructed using tablet-shaped samples and an original mounting base.

    • Main Results:

    • The tissue tablet method demonstrated no significant difference in tissue quality compared to conventional OCT blocks.
    • Constructed frozen tissue microarrays exhibited good morphology, minimal artifacts, and successful immunohistochemistry and in situ hybridization staining.
    • The method proved suitable for creating high-quality frozen tissue microarrays.

    Conclusions:

    • The tissue tablet method offers an efficient, time-saving, and user-friendly approach for freezing and storing human tissues.
    • This method enhances tissue handling and processing, meeting the demands of modern research and tissue banking.
    • The developed procedure facilitates the creation of high-quality frozen tissue microarrays suitable for various research applications.