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Genotypic variation among six isolates of lactate dehydrogenase-elevating virus
The Journal of General Virology
|December 1, 1986
Summary
Multiple genotypes of lactate dehydrogenase-elevating virus (LDV) exist, as shown by RNA fingerprinting. LDV possesses a 3'-terminal poly(A) tract, supporting its classification within the Togaviridae family.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Lactate dehydrogenase-elevating virus (LDV) is an economically important pathogen in the swine industry.
- Understanding LDV genetic diversity is crucial for developing effective control strategies.
Purpose of the Study:
- To investigate the genetic diversity among different LDV isolates.
- To confirm the presence of a 3'-terminal poly(A) tract in LDV RNA.
- To re-evaluate LDV's taxonomic classification.
Main Methods:
- RNA oligonucleotide fingerprint analysis of six independent LDV isolates.
- Direct sequencing of 3'-32P-labeled LDV genome RNA.
Main Results:
- Four out of six LDV isolates exhibited unique RNA fingerprint patterns.
- Two isolates showed patterns similar but not identical to one of the unique patterns, indicating distinct genotypes.
- A 3'-terminal poly(A) tract was confirmed in the LDV genome RNA.
Conclusions:
- The findings demonstrate the existence of multiple LDV genotypes.
- LDV isolates can be categorized based on their genetic profiles.
- The presence of a 3'-terminal poly(A) tract supports LDV's classification within the Togaviridae family, differentiating it from Flaviviridae.