In vivo x-ray luminescence tomographic imaging with single-view data

Area of Science:

• Biomedical Imaging
• Nanotechnology
• Medical Physics

Background:

• X-ray luminescence computed tomography (XLCT) is an emerging molecular imaging technique.
• Near-infrared emitting nanophosphors, such as Gd2O3:Eu3+, are promising for in vivo imaging applications.

Purpose of the Study:

• To demonstrate the feasibility of in vivo XLCT imaging using single-view data.
• To evaluate the accuracy and efficiency of a compressive sensing-based XLCT reconstruction method.
• To assess the potential of XLCT for imaging fast distribution of nanophosphors in biological systems.

Main Methods:

• In vivo tomographic imaging of Gd2O3:Eu3+ nanophosphors in nude mice.
• Application of compressive sensing techniques for XLCT reconstruction from single-view data.
• Quantitative analysis of location error and imaging time.

Main Results:

• Successful in vivo tomographic imaging of nanophosphors in nude mice was achieved.
• Single-view XLCT reconstruction demonstrated feasibility with a location error below 1.5 mm.
• Imaging time was significantly reduced compared to previous XLCT systems.

Conclusions:

• Single-view XLCT reconstruction is a viable and efficient method for in vivo molecular imaging.
• The reduced imaging time makes XLCT suitable for dynamic studies of nanophosphor distribution.
• This technique holds promise for advancing biological and medical research.