Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Imaging01:24

X-ray Imaging

7.7K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
7.7K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D virtual histopathology of human breast tissues using an X-ray grating interferometry micro CT system.

Experimental and molecular pathology·2026
Same author

An achromatic neutron lens.

Nature communications·2026
Same author

Histology-guided 3D virtual staining of microCT-imaged lung tissue via deep learning.

Journal of the Royal Society, Interface·2026
Same author

Paired CycleGAN-based virtual staining for 3D X-ray histology of bone-implant systems.

Journal of synchrotron radiation·2026
Same author

Spin Waves Excited by Hard X-Ray Transient Gratings.

Physical review letters·2026
Same author

Directional dark field for nanoscale full-field transmission X-ray microscopy.

Light, science & applications·2026

Related Experiment Video

Updated: May 5, 2026

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph
05:32

Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

Published on: February 21, 2025

747

A sensitive x-ray phase contrast technique for rapid imaging using a single phase grid analyzer.

Kaye S Morgan, Peter Modregger, Sarah C Irvine

    Optics Letters
    |December 11, 2013
    PubMed
    Summary

    This study introduces a fast phase contrast x-ray imaging (PCXI) method using a single exposure. This technique enables high-speed imaging and live animal studies with improved sensitivity and reduced radiation dose.

    More Related Videos

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    9.0K
    Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
    10:39

    Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

    Published on: October 11, 2016

    9.1K

    Related Experiment Videos

    Last Updated: May 5, 2026

    Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph
    05:32

    Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph

    Published on: February 21, 2025

    747
    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography
    10:18

    Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

    Published on: February 21, 2017

    9.0K
    Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
    10:39

    Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

    Published on: October 11, 2016

    9.1K

    Area of Science:

    • Medical Imaging
    • Biophysics
    • X-ray Optics

    Background:

    • Phase contrast x-ray imaging (PCXI) offers high soft tissue contrast and spatial resolution.
    • Current PCXI methods often require long exposure times or multiple exposures, limiting speed and increasing radiation dose.

    Purpose of the Study:

    • To develop a high-speed PCXI method using a single, short exposure.
    • To enable real-time imaging applications, including live animal studies.

    Main Methods:

    • Illumination with a checkerboard phase grid to create a reference intensity pattern.
    • Mapping sample-induced distortions of the pattern to recover differential phase images.
    • Retrieval of five distinct image types from a single exposure.

    Main Results:

    • Demonstrated a PCXI method with a single short exposure for high-speed imaging.
    • Achieved minimal loss in x-ray flux and a fine pattern for high spatial resolution.
    • Successfully retrieved sample phase gradient (horizontal/vertical), projected phase depth, edge-enhanced, and scattering images.

    Conclusions:

    • The developed PCXI technique significantly enhances imaging speed without compromising sensitivity or spatial resolution.
    • The method allows for high-speed x-ray movies and live animal imaging.
    • The reconstruction technique supports subpixel distortion retrieval and analysis of imaging parameters.