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Wide-field two-dimensional multifocal optical-resolution photoacoustic-computed microscopy.

Jun Xia, Guo Li, Lidai Wang

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    |December 11, 2013
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    We developed 2D multifocal optical-resolution photoacoustic-computed microscopy (2D-MFOR-PACM) for faster, high-resolution imaging. This new system significantly reduces imaging time and improves resolution compared to previous methods.

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    Area of Science:

    • Biomedical Optics
    • Photoacoustics
    • Microscopy

    Background:

    • Optical-resolution photoacoustic microscopy (OR-PAM) offers high-resolution optical absorption imaging in tissues.
    • Existing OR-PAM systems often use single-focused excitation, limiting wide-field imaging speed.
    • Previous multifocal approaches (1D-MFOR-PAM) have not fully utilized microlens and transducer array potential.

    Purpose of the Study:

    • To develop a novel 2D multifocal optical-resolution photoacoustic-computed microscopy (2D-MFOR-PACM) system.
    • To enhance imaging speed and spatial resolution in photoacoustic microscopy.
    • To demonstrate the system's capability for imaging phantoms and biological samples.

    Main Methods:

    • Utilized a 2D microlens array and a full-ring 512-element ultrasonic transducer array.
    • Generated 1800 optical foci across a 10 mm×10 mm field of view.
    • Employed raster scanning of the microlens array for image acquisition.

    Main Results:

    • Achieved an in-plane resolution of 29 μm, a significant improvement from ≥100 μm.
    • Completed imaging of a 10 mm×10 mm field of view in just 36 seconds.
    • Demonstrated superior speed compared to 1D-MFOR-PAM, which requires over 4 minutes for the same area.

    Conclusions:

    • The developed 2D-MFOR-PACM system substantially increases photoacoustic imaging speed and resolution.
    • This advancement overcomes limitations of previous OR-PAM techniques.
    • The system shows promise for various biomedical imaging applications in phantoms and live subjects.