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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: May 5, 2026

Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor
07:28

Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor

Published on: August 30, 2012

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Local terahertz microspectroscopy with λ/100 spatial resolution.

F Glotin, J-M Ortega, R Prazeres

    Optics Letters
    |December 11, 2013
    PubMed
    Summary
    This summary is machine-generated.

    Researchers extended infrared microspectroscopy to the terahertz region, achieving high spatial resolution for chemical mapping. This technique offers detailed analysis of materials like graphite-polymer composites.

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    Area of Science:

    • Optics and Spectroscopy
    • Materials Science
    • Chemical Analysis

    Background:

    • Infrared microspectroscopy is a powerful tool for chemical analysis.
    • Extending its spectral range enables the study of new material properties.

    Purpose of the Study:

    • To adapt differential infrared microspectroscopy for terahertz (THz) spectral region operation.
    • To evaluate the spatial resolution and chemical mapping capabilities of the extended method.

    Main Methods:

    • Differential infrared microspectroscopy was modified to cover the terahertz spectral range.
    • Experiments were conducted on graphite embedded in polymer matrices.

    Main Results:

    • The spatial resolution achieved was practically independent of wavelength.
    • A spatial resolution of at least λ/100 was demonstrated.
    • The method showed sensitivity to the imaginary part of the refractive index.

    Conclusions:

    • The adapted terahertz infrared microspectroscopy method enables high-resolution chemical mapping.
    • This technique is suitable for analyzing the composition of various materials, including composites.