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Updated: May 5, 2026
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
Published on: December 24, 2014
Construction and optimization of a Pseudomonas putida whole-cell bioreporter for detection of bioavailable copper
Peng-Song Li1, Zhi-Wen Peng, Jie Su
1Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen, 518055, China, lips@pku.edu.cn.
Abstract:
A Pseudomonas putida whole-cell bioreporter for detecting bioavailable copper was constructed by inserting a CueR-regulated sensor element upstream of a promoterless green fluorescent protein (GFP) reporter gene. The constructed bioreporter cells expressed GFP only in response to Cu and Ag when cultivated in different metal salt solutions. M9 supplemented medium provided higher sensitivity compared with LB medium. The optimal test condition was cell suspension with an OD600 of 0.4-0.5 incubated at 30 °C. The detection range of Cu is 1-70 mg/l under optimal test condition in M9 supplemented medium.
