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Ischemic Stroke ll: Pathophysiology

Ischemic Stroke ll: Pathophysiology

An ischemic stroke occurs when a cerebral blood vessel becomes obstructed, most often by a thrombus or embolus, interrupting the delivery of oxygen and glucose to brain tissue. Because neurons rely on continuous aerobic metabolism, energy failure begins within minutes of reduced perfusion. The region receiving the least blood flow becomes the infarct core, an area of irreversible cellular death. Surrounding this core lies the penumbra, a zone of hypoperfused but still viable tissue that is...

Ischemic Stroke l: Introduction

Ischemic Stroke l: Introduction

Ischemic stroke is an acute cerebrovascular condition in which blood flow to a brain region is suddenly interrupted, leading to tissue infarction. Neurons depend on continuous oxygen and glucose supply, so even brief reductions in perfusion cause energy failure, ionic imbalance, and irreversible injury. Ischemic strokes are classified into thrombotic and embolic types based on their underlying mechanisms.Thrombotic MechanismsThrombotic stroke develops when a clot forms within a cerebral artery.

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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

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MicroRNAs in Cerebral Ischemia.

Kai-Ying Lim¹, Jia-Hui Chua, Jun-Rong Tan

¹Department of Biochemistry, The Stroke Program, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 8 Medical Drive, Singapore, 117597, Singapore.

Translational Stroke Research

|December 11, 2013

Summary

This study reveals microRNAs (miRNAs) as potential early biomarkers for cerebral ischemia. Profiling miRNAs in rat models shows their dynamic changes during stroke progression, offering new insights beyond protein biomarkers.

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Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Cerebral ischemia pathogenesis traditionally focuses on proteins and their regulated pathways.
Current biomarker discovery primarily targets proteins and their encoding mRNAs.
The role of microRNAs (miRNAs) in cerebral ischemia is an emerging area of research.

Purpose of the Study:

To profile microRNAs (miRNAs) during the progression of cerebral ischemia in a rat model.
To investigate the correlation between miRNA expression, NMDA antagonist treatment (MK801), and protein expression.
To explore the potential of miRNAs as early biomarkers for stroke.

Main Methods:

Utilized rat models subjected to middle cerebral artery occlusion (MCAO).
Performed microRNA profiling at various reperfusion times (0-48 hours).
Correlated miRNA expression with NMDA antagonist treatment and protein levels.

Main Results:

Identified dynamic changes in microRNA expression profiles following cerebral ischemia.
Demonstrated correlations between specific miRNAs, therapeutic intervention (MK801), and protein expression patterns.
Provided evidence for the potential utility of miRNAs in understanding stroke pathogenesis.

Conclusions:

MicroRNAs represent a novel class of potential biomarkers for cerebral ischemia.
Understanding miRNA dynamics offers new perspectives on stroke progression and treatment response.
Further research into miRNAs could significantly advance early stroke detection and therapeutic strategies.