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[Optimal time for MRI scanning of tumors in BALB/c mice using c-erbB2 antisense probe labeled with superparamagnetic
Zhi-Peng Wen1, Hai-Yan Liu, Ming Wen
1Department of Radiology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Objective:
To determine the optimal time for MRI scanning of tumors in BALB/c mice using c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles.
Methods:
SK-Br-3 tumor-bearing BALB/c mice (n= 30) were injected with antisense probe (12.0 mg Fe/kg). MRI scanning was performed on 5 mice before the injection and 60 min, 180 min, 360 min, 720 min and 1 440 min after the injection, respectively. Tumor tissues were taken immediately after the scanning and fixed with 10% formalin and paraffin-embedded sections. The MRI signal strength of the tumors and adjacent muscles were compared with changes detected under a microscope using HE and Prussian blue staining.
Results:
SK-Br-3 tumors were introduced to the BALB/c mice successfully. The strongest signal intensity was detected by the MRI 360 min after injection with the antisense probe. The pathological examination revealed structural disorders of the tumor issues, with a large number of special-shaped cells arranged in a cancer nest shape. Punctuate blue iron particles were observed in all of the tumor issues, with the greatest density occurring at 360 min after the injection with the antisense probe.
Conclusion:
The MR4 optimal time for MRI scanning of tumors in BALB/c mice using c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles should be set at 360 min after injection.
Insights
Optimal MRI scanning time for c-erbB2 tumors in mice using superparamagnetic iron oxide nanoparticles is 360 minutes post-injection. This timing maximizes signal intensity for accurate tumor detection and characterization.
Area of Science:
- Biomedical Imaging
- Nanotechnology in Medicine
- Oncology Research
Background:
- Superparamagnetic iron oxide nanoparticles (SPIONs) enhance MRI contrast.
- Antisense probes target specific gene expressions, like c-erbB2 in tumors.
- Optimizing imaging timing is crucial for accurate diagnostic and research outcomes.
Purpose of the Study:
- To determine the optimal time for MRI scanning of c-erbB2 tumors in BALB/c mice.
- To evaluate the efficacy of c-erbB2 antisense probe labeled with SPIONs for tumor imaging.
- To correlate MRI signal intensity with pathological findings.
Main Methods:
- SK-Br-3 tumor-bearing BALB/c mice were injected with a c-erbB2 antisense probe labeled with SPIONs.
- MRI scans were performed at multiple time points post-injection (60, 180, 360, 720, 1440 minutes).
- Tumor tissues were analyzed via HE and Prussian blue staining to assess iron particle distribution and tumor morphology.
Main Results:
- The highest MRI signal intensity was observed at 360 minutes post-injection.
- Pathological examination confirmed tumor presence and revealed structural abnormalities.
- Prussian blue staining showed the greatest density of iron particles in tumor tissues at 360 minutes.
Conclusions:
- 360 minutes post-injection represents the optimal time for MRI scanning of c-erbB2 tumors using SPION-labeled antisense probes.
- This optimized timing enhances the detection and characterization of tumors.
- The findings support the use of SPION-conjugated antisense probes for targeted tumor imaging in preclinical models.
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