Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liquid Atmospheric Pressure MALDI (LAP-MALDI) Mass Spectrometry: A Versatile Tool for the Characterization of Synthetic Polymers.

Rapid communications in mass spectrometry : RCM·2026
Same author

Correction to: Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry.

Analytical and bioanalytical chemistry·2025
Same author

Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry.

Analytical and bioanalytical chemistry·2025
Same author

Rapid and accurate biological sex estimation by LAP-MALDI MS analysis of child teeth.

The Analyst·2025
Same author

LAP-MALDI MS analysis of amelogenin from teeth for biological sex estimation.

Journal of pharmaceutical and biomedical analysis·2024
Same author

Detection of Antimicrobial Proteins/Peptides and Bacterial Proteins Involved in Antimicrobial Resistance in Raw Cow's Milk from Different Breeds.

Antibiotics (Basel, Switzerland)·2024

Related Experiment Video

Updated: May 5, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

10.8K

Enhanced MALDI MS sensitivity by weak base additives and glycerol sample coating.

Rainer Cramer1, Michael Karas, Thorsten W Jaskolla

  • 1Department of Chemistry, University of Reading , Whiteknights, Reading RG6 6AD, United Kingdom.

Analytical Chemistry
|December 12, 2013
PubMed
Summary

Rationally designing MALDI matrices improved sensitivity by avoiding excess laser absorption additives. Adding proton donors and glycerol significantly enhanced matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) performance.

More Related Videos

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

779
Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

952

Related Experiment Videos

Last Updated: May 5, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

10.8K
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
08:51

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Published on: June 20, 2025

779
Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

952

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is a powerful analytical technique.
  • Improving MALDI MS sensitivity is crucial for detecting low-abundance analytes.
  • Current MALDI matrix design focuses on analyte co-crystallization.

Purpose of the Study:

  • To extend rational MALDI matrix design to a "whole sample" level.
  • To identify key factors influencing MALDI MS sensitivity.
  • To gain new mechanistic insights into MALDI processes.

Main Methods:

  • Investigated the effect of various additives on MALDI MS performance.
  • Evaluated additives based on laser energy absorption, proton donation, and sample coating properties.
  • Compared analytical sensitivity against established MALDI MS standards.

Main Results:

  • Additives solely increasing laser energy absorption were detrimental.
  • Proton donors, specifically protonated weak bases, significantly improved sensitivity.
  • Glycerol addition was highly effective for coating crystalline samples.
  • Overall analytical sensitivity was substantially increased compared to current "gold" standards.

Conclusions:

  • Rational "whole sample" matrix design offers significant improvements in MALDI MS sensitivity.
  • Understanding additive roles (energy absorption, proton donation, coating) is key to optimizing MALDI.
  • New mechanistic insights into MALDI processes have been elucidated.