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Related Experiment Video

Updated: May 5, 2026

Providing Meaningful Environmental Enrichment and Measuring Saliva Cortisol in Pigs Housed on Slatted Flooring
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Collection of Oral Fluids Using Cotton Ropes as a Sampling Method to Detect Foot-and-Mouth Disease Virus Infection in

W Vosloo1, J Morris1, A Davis1

  • 1CSIRO-Australian Animal Health Laboratory, Geelong, Vic., Australia.

Transboundary and Emerging Diseases
|December 12, 2013
PubMed
Summary
This summary is machine-generated.

Rope-collected oral fluid samples offer a sensitive and non-invasive method for detecting foot-and-mouth disease (FMD) virus in pigs. This cost-effective approach aids in monitoring high-density farms and preventing rapid disease spread.

Keywords:
diagnosisfoot-and-mouth disease viruspigsrope

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Area of Science:

  • Veterinary Virology
  • Animal Health Monitoring
  • Infectious Disease Diagnostics

Background:

  • High-density pig farming necessitates robust monitoring for rapidly spreading infectious diseases.
  • Foot-and-mouth disease (FMD) poses a significant threat due to pigs' ability to amplify and spread the virus.
  • Early and accurate detection of FMD virus is crucial for disease control and minimizing transmission.

Purpose of the Study:

  • To evaluate the efficacy of using ropes for collecting oral fluid samples for FMD virus detection in pigs.
  • To compare the sensitivity and agreement of rope sampling with traditional individual saliva sampling.
  • To assess the potential of rope sampling as a cost-effective, non-invasive diagnostic tool for FMD surveillance.

Main Methods:

  • Oral fluid samples were collected from pigs using ropes.
  • FMD virus RNA was detected in collected samples using real-time PCR.
  • Two experiments involved infected and unvaccinated pigs, with some in aerosol contact, using different FMD virus serotypes.
  • Rope sample results were compared against individual saliva sample results.

Main Results:

  • The sensitivity of rope sampling for FMD virus detection ranged from 0.67 to 0.92.
  • Statistical agreement between rope sampling and individual sampling was substantial to moderate across different serotypes.
  • The method demonstrated high sensitivity for detecting FMD virus RNA.

Conclusions:

  • Rope-based oral fluid sampling is a sensitive and practical method for monitoring FMD virus in pig populations.
  • This non-invasive technique offers a potentially cost-effective alternative to individual sampling for FMD surveillance.
  • Further validation of RNA detection sensitivity could establish this as a valuable diagnostic tool.