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Updated: May 5, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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YY1 represses the transcriptional activity of Runx2 in C2C12 cells

Hyung Min Jeong1, You Hee Choi1, Sung Ho Lee1

  • 1College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, South Korea.

Molecular and Cellular Endocrinology
|December 12, 2013
PubMed

Insights

Transcription factor YY1 inhibits osteoblast differentiation by interacting with Runx2, decreasing its DNA binding and transcriptional activity. Knocking down YY1 enhances differentiation, revealing YY1

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Runx2 is a key transcription factor for osteoblast differentiation induced by bone morphogenetic proteins (BMPs).
  • YY1 is a transcription factor known to inhibit BMP2-induced cell differentiation.
  • The regulatory relationship between Runx2 and YY1 in osteoblast differentiation was previously unclear.

Purpose of the Study:

  • To investigate the interaction and regulatory relationship between Runx2 and YY1 in osteoblast differentiation.
  • To elucidate the mechanism by which YY1 affects Runx2 activity.

Main Methods:

  • Confirmation of alkaline phosphatase staining repression by YY1.
  • Analysis of Runx2 and YY1 interaction domains.
  • Assessment of transcriptional activity on osteocalcin and alkaline phosphatase promoters.
  • Evaluation of osteoblast differentiation upon YY1 knockdown.
  • Measurement of Runx2 DNA binding affinity.

Main Results:

  • YY1 was confirmed to repress alkaline phosphatase staining.
  • Runx2 and YY1 interact via the Runt domain and C-terminus of Runx2.
  • YY1 significantly repressed Runx2-mediated transcriptional enhancement.
  • Knockdown of YY1 promoted BMP2- and Runx2-induced osteoblast differentiation.
  • YY1 decreased the DNA binding affinity of Runx2.

Conclusions:

  • YY1 represses osteoblast differentiation through direct interaction with Runx2.
  • YY1 inhibits the transcriptional activity of Runx2, thereby suppressing osteoblast differentiation.
  • Understanding this interaction provides insights into the regulation of bone formation.
Keywords:
DNA binding affinityRunx2Transcriptional activityYY1

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