Related Experiment Video
Updated: May 5, 2026

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
Published on: June 14, 2021
Vitreous base visualisation through trans-scleral illumination with a standard 25-gauge light probe
Giulio Bamonte1, Pieter R van den Biesen
1Department of Ophthalmology, Hagaziekenhuis, , The Hague, The Netherlands.
Purpose:
To describe a technique of vitreous base visualisation through trans-scleral illumination using a standard 25-gauge light probe.
Methods:
All vitrectomies are performed using 25-gauge+ instruments and valved trocars. A non-contact viewing system is used to visualise the retina. After core vitrectomy and the necessary additional procedures, triamcinolone acetonide (Kenacort) is injected in the vitreous cavity. Then, the standard 25-gauge light pipe is covered with a sleeve obtained from a 20-gauge venflon cannula. The light brightness is increased to 100%, and the light probe used to indent the sclera and trans-illuminate the vitreous base. The vitreous cutter is activated between the crystals of triamcinolone acetonide and the retinal surface. Complete vitreous base shaving is carried out for 360°.
Results:
Iatrogenic peripheral retinal tears, as a result of vitreous shaving, occurred in 4.1% of cases with this technique.
Conclusions:
This method represents a valid and low-cost option to achieve accurate vitreous base shaving.

