Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

4.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resident T cell activation leads to human hair follicle immune privilege loss <i>ex vivo</i>, which is prevented by the DHODH inhibitor farudodstat: relevance for alopecia areata.

Frontiers in medicine·2026
Same author

Toward cell-based therapy of alopecia areata: Autologous human Vδ2<sup>+</sup> Foxp3<sup>+</sup> γδTreg cells restore hair-follicle immune privilege and promote hair regrowth in human alopecia areata models ex vivo and in vivo.

The Journal of investigative dermatology·2026
Same author

The Wonders of Humanized Mouse Models for Skin Disease and Research.

The Israel Medical Association journal : IMAJ·2025
Same author

Preclinical Evidence That Mesoglycan Unfolds Complex Anti-Aging Effects in Photoaged Female Facial Skin.

International journal of molecular sciences·2025
Same author

Lessons from TNF-α Inhibitor-Induced Alopecia Areata: Does TNF-α Operate as a Hair Follicle Immune Privilege Guardian under Inflammatory Conditions?

The Journal of investigative dermatology·2025
Same author

Topical Minoxidil Rejuvenates Hair Follicles from Men with Androgenetic Alopecia in Vivo.

Acta dermato-venereologica·2024

Related Experiment Video

Updated: May 4, 2026

A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis
06:41

A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis

Published on: March 9, 2015

8.7K

A new humanized mouse model for alopecia areata.

Amos Gilhar1, Aviad Keren, Ralf Paus

  • 1Skin Research Laboratory, Faculty of Medicine, Technion-Israel Institute of Technology and Flieman Medical Center, Haifa, Israel.

The Journal of Investigative Dermatology. Symposium Proceedings
|December 12, 2013
PubMed
Summary

A new humanized mouse model for alopecia areata (AA) was developed. This model highlights the role of specific immune cells in hair loss, aiding the search for effective treatments.

More Related Videos

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
05:15

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery

Published on: September 27, 2021

1.1K
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

10.5K

Related Experiment Videos

Last Updated: May 4, 2026

A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis
06:41

A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis

Published on: March 9, 2015

8.7K
Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
05:15

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery

Published on: September 27, 2021

1.1K
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

10.5K

Area of Science:

  • Immunology
  • Dermatology
  • Autoimmune Diseases

Background:

  • Alopecia areata (AA) is an organ-specific autoimmune disease causing significant psychological distress.
  • Current understanding of AA pathogenesis is limited, necessitating improved animal models for research.
  • There is a need for effective therapeutic strategies to manage AA and improve patients' quality of life.

Purpose of the Study:

  • To develop a novel humanized mouse model for studying alopecia areata (AA).
  • To investigate the role of specific immune cells in the pathogenesis of AA.
  • To provide a platform for identifying and testing new therapeutic strategies for AA.

Main Methods:

  • Human scalp skin from healthy volunteers was transplanted onto severe-combined immunodeficient (SCID) mice.
  • Peripheral blood mononuclear cells (PBMCs) enriched for NKG2D+ and CD56+ cells were cultured with IL-2 and injected intradermally.
  • The development of AA was monitored through clinical, histological, and immunohistochemical analysis.

Main Results:

  • The humanized mouse model exhibited rapid and predictable focal hair loss.
  • The induced hair loss mirrored the characteristic features of human alopecia areata.
  • The model demonstrated the functional importance of NKG2D+ and CD56+ cells in AA pathogenesis.

Conclusions:

  • A new, reliable humanized mouse model for alopecia areata has been successfully established.
  • This model confirms the critical role of NKG2D+ and CD56+ cells in the development of AA.
  • The model holds promise for advancing the understanding and treatment of alopecia areata.