Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Forced Transdifferentiation01:28

Forced Transdifferentiation

1.5K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.5K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A robust and user-agnostic step-emulsion platform for scalable microgel fabrication.

bioRxiv : the preprint server for biology·2026
Same author

Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.

ACS biomaterials science & engineering·2026
Same author

Amoeboid-mesenchymal transition and the proteolytic control of cancer invasion plasticity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Amoeboid-Mesenchymal Transition and the Proteolytic Control of Cancer Invasion Plasticity.

bioRxiv : the preprint server for biology·2025
Same author

PEG-Collagen Interpenetrating Networks Support Enhanced Vasculogenic Self-Assembly and Impact Cell-Mediated Remodeling.

ACS biomaterials science & engineering·2025
Same author

Elasticity of fibers prefers the chaos of turbulence.

Physical review. E·2025

Related Experiment Video

Updated: May 4, 2026

Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

790

Matrix identity and tractional forces influence indirect cardiac reprogramming.

Yen P Kong1, Bita Carrion, Rahul K Singh

  • 1Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, Michigan, 48109, USA.

Scientific Reports
|December 12, 2013
PubMed
Summary

In vivo cardiac reprogramming shows promise for heart regeneration. Extracellular microenvironment factors, like matrix identity and cell forces, are key to improving reprogramming efficiency for better heart repair outcomes.

More Related Videos

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.2K
Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

6.8K

Related Experiment Videos

Last Updated: May 4, 2026

Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

790
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.2K
Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

6.8K

Area of Science:

  • Cardiovascular research
  • Regenerative medicine
  • Biomaterials science

Background:

  • In vivo cardiac reprogramming is a promising strategy for heart regeneration.
  • Understanding the extracellular microenvironment's role is crucial for enhancing reprogramming efficiency.

Purpose of the Study:

  • To identify key determinants of the extracellular microenvironment influencing cardiac reprogramming.
  • To elucidate the roles of matrix properties and cell-generated forces in cardiac reprogramming stages.

Main Methods:

  • Investigated the impact of matrix identity and cell-generated tractional forces on cardiac reprogramming.
  • Assessed the influence of cell proliferation, matrix mechanics, and microstructure.

Main Results:

  • Matrix identity and cell-generated tractional forces were identified as primary drivers of dedifferentiation and differentiation during reprogramming.
  • Cell proliferation, matrix mechanics, and microstructure showed secondary influences.

Conclusions:

  • The extracellular microenvironment significantly impacts in vivo cardiac reprogramming efficiency.
  • Optimizing matrix properties and cell-generated forces can enhance cardiac regeneration strategies.