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Related Experiment Video

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Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models

Author Spotlight: Advancing Tendon Tissue Engineering with 3D Organoid Models

Published on: June 21, 2024

Human collagen-based multilayer scaffolds for tendon-to-bone interface tissue engineering.

Beob Soo Kim¹, Eun Ji Kim, Ji Suk Choi

¹Department of Chemical Engineering, Hanyang University, Hanyangdaehak-ro 55, Ansan, Kyeonggi-do 426-791, Republic of Korea; Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seoul, 136-791, Republic of Korea.

Journal of Biomedical Materials Research. Part A

|December 12, 2013

Summary

Researchers created a human collagen scaffold with four layers to mimic the natural tendon-to-bone transition. This scaffold supports cell growth and shows promise for regenerating complex connective tissues.

Keywords:

adipose tissue calcification collagen fibrocartilage tendon-to-bone

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Last Updated: May 4, 2026

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Published on: June 21, 2024

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Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

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Published on: July 6, 2022

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

The natural tendon-to-bone interface exhibits a complex gradient of properties crucial for its function.
Natural healing processes often fail to fully restore this intricate transitional tissue.
Existing regenerative strategies struggle to replicate the native gradient structure.

Purpose of the Study:

To develop a novel multilayer scaffold that mimics the gradient properties of the native tendon-to-bone interface.
To evaluate the scaffold's ability to support the adhesion and proliferation of relevant cell types.

Main Methods:

Fabrication of a four-layer scaffold using human collagen with varying compositions (collagen, chondroitin sulfate, apatite).
Characterization of scaffold properties using scanning electron microscopy, porosimetry, tensile testing, FTIR, EDX, and TGA.
Assessment of cell adhesion and proliferation (fibroblasts, chondrocytes, osteoblasts) on different scaffold layers.

Main Results:

The scaffold successfully replicated the compositional and structural gradient of the native tendon-to-bone region.
Characterization confirmed a gradual transition in chemical, physical, and mechanical properties across the scaffold layers.
The scaffold supported site-specific adhesion and proliferation of human fibroblasts, chondrocytes, and osteoblasts.

Conclusions:

A human collagen-based multilayer scaffold can effectively mimic the tendon-to-bone interface.
The developed scaffold demonstrates potential for regenerating hard-to-soft interface tissues.
This approach offers a promising strategy for addressing limitations in current regenerative medicine techniques.