Related Experiment Video

Updated: May 4, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

28.0K

A new regulatory pathway of mRNA export by an F-box protein, Mdm30

Geetha Durairaj, Shweta Lahudkar, Sukesh R Bhaumik

    RNA (New York, N.Y.)
    |December 12, 2013
    PubMed

    Insights

    Mdm30 protein promotes mRNA export in yeast by targeting Sub2 for degradation. This process enhances the recruitment of Yra1, a key mRNA export adaptor, revealing a novel regulatory mechanism.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Mdm30, an F-box protein, is known to promote mRNA export in yeast.
    • The precise mechanism by which Mdm30 facilitates mRNA export remains unclear.

    Purpose of the Study:

    • To elucidate the mechanism through which Mdm30 promotes mRNA export.
    • To investigate the interaction between Mdm30 and the mRNA export machinery.

    Main Methods:

    • Ubiquitylation assays to detect Sub2 modification.
    • Proteasomal degradation assays to assess Sub2 stability.
    • Analysis of Yra1 recruitment to active genes using techniques like ChIP-seq.

    Main Results:

    • Mdm30 targets the Sub2 protein, a component of the TREX complex, for ubiquitylation.
    • Ubiquitylation leads to the proteasomal degradation of Sub2.
    • The degradation of Sub2 enhances the recruitment of the mRNA export adaptor Yra1 to active genes.

    Conclusions:

    • Mdm30 promotes mRNA export by reducing Sub2 stability.
    • This reduction in Sub2 stability facilitates enhanced Yra1 recruitment.
    • Mdm30 regulates mRNA export through a novel mechanism involving Sub2 degradation and Yra1 enhancement.
    Keywords:
    Mdm30Sub2TREXYra1mRNA exporttranscription

    More Related Videos

    Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells10:24

    Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

    Published on: December 17, 2012

    13.5K
    Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection11:32

    Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

    Published on: December 4, 2010

    17.1K

    Related Concept Videos

    Regulated mRNA Transport02:22

    Regulated mRNA Transport

    In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
    5.7K
    Regulated mRNA Transport02:22

    Regulated mRNA Transport

    2.5K
    Nuclear Export01:42

    Nuclear Export

    The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
    NES are of three types- the canonical 10-residue long leucine-rich signal and other...
    3.7K
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
    7.1K
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    4.7K
    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
    Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
    9.4K