Related Experiment Video

Updated: May 4, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

7.1K

AKT kinase pathway: a leading target in cancer research

Ambuj Kumar1, Vidya Rajendran, Rao Sethumadhavan

  • 1Bioinformatics Division, School of Bio Sciences and Technology, Vellore Institute of Technology University, Vellore, Tamil Nadu 632014, India.

Thescientificworldjournal
|December 12, 2013
PubMed

Insights

This study explores targeting AKT kinase and miR-21 for cancer therapy. Doxorubicin and increased FOXO3A show promise in inhibiting cancer cell proliferation via these pathways.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cancer Research

Background:

  • The AKT kinase pathway is crucial for cell proliferation, survival, and growth.
  • Targeting AKT kinases for cancer therapy has shown limited success despite numerous attempts.
  • MicroRNA-21 (miR-21) plays a significant role in cancer progression.

Purpose of the Study:

  • To provide a detailed molecular insight into AKT kinase protein.
  • To propose a doxorubicin-based strategy for inhibiting miR-21-mediated cancer cell proliferation.
  • To investigate the potential of increasing FOXO3A concentration to reduce miR-21 activity.

Main Methods:

  • Molecular analysis of AKT kinase protein.
  • Computational drug design approaches.
  • Next-generation sequencing techniques.

Main Results:

  • A doxorubicin-based approach is proposed for inhibiting miR-21-driven cancer cell proliferation.
  • Elevating FOXO3A concentration shows potential in reducing miR-21-mediated cancer activation.
  • Next-generation sequencing and computational drug design are identified as valuable tools.

Conclusions:

  • Targeting the AKT/miR-21 axis presents a promising avenue for cancer treatment.
  • FOXO3A modulation offers a potential strategy to counteract miR-21's oncogenic effects.
  • Advanced sequencing and computational methods are essential for developing novel anti-cancer drugs.

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

951
A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors12:40

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

Published on: December 7, 2014

14.5K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.5K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.4K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.3K