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Biologically active domain in somatomedin-binding protein
Biochemical and Biophysical Research Communications
|November 26, 1986
Summary
Human decidua synthesizes somatomedin-binding protein PP12. Researchers identified a 21K fragment, suggesting it contains the somatomedin-binding domain and is the N-terminal portion of the protein.
Area of Science:
- Reproductive biology
- Endocrinology
- Protein biochemistry
Background:
- Human decidua and placenta synthesize a 34K somatomedin-binding protein, termed PP12.
- Immunochemical purification yielded lower-molecular weight immunoreactive polypeptides (PP12B).
Purpose of the Study:
- To characterize the structure and function of PP12 and its fragments.
- To identify the somatomedin-binding domain of PP12.
Main Methods:
- Immunochemical purification
- High-performance liquid chromatography (HPLC)
- Western blotting
- N-terminal amino acid sequencing
Main Results:
- Isolated a 21K fragment and a mixture of 17-20K fragments from PP12B.
- These fragments reacted with antibodies to native PP12.
- The 21K fragment shares the N-terminal amino acid sequence with PP12 and binds somatomedin-C/IGF-I.
- The N-terminal end of the 21K fragment is identical to that of the 34K PP12 protein.
Conclusions:
- The 21K fragment represents the N-terminal portion of the 34K somatomedin-binding protein PP12.
- The somatomedin-binding domain is located within this N-terminal 21K fragment.