Related Experiment Video
Updated: May 4, 2026

In situ TEM of Biological Assemblies in Liquid
Published on: December 30, 2013
Improved microchip design and application for in situ transmission electron microscopy of macromolecules
Madeline J Dukes1, Rebecca Thomas1, John Damiano1
11 Protochips Inc., Raleigh, NC 27606, USA.
Abstract:
Understanding the fundamental properties of macromolecules has enhanced the development of emerging technologies used to improve biomedical research. Currently, there is a critical need for innovative platforms that can illuminate the function of biomedical reagents in a native environment. To address this need, we have developed an in situ approach to visualize the dynamic behavior of biomedically relevant macromolecules at the nanoscale. Newly designed silicon nitride devices containing integrated "microwells" were used to enclose active macromolecular specimens in liquid for transmission electron microscopy imaging purposes.We were able to successfully examine novel magnetic resonance imaging contrast reagents, micelle suspensions, liposome carrier vehicles, and transcribing viral assemblies. With each specimen tested, the integrated microwells adequately maintained macromolecules in discrete local environments while enabling thin liquid layers to be produced.
Related Concept Videos
Transmission Electron Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Immunogold Electron Microscopy
Overview of Electron Microscopy
Cryo-electron Microscopy
Preparation of Samples for Electron Microscopy

