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Updated: May 4, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Standardization of Tc-99 by three liquid scintillation counting methods.

W M van Wyngaardt1, M J van Staden1, J Lubbe1

  • 1National Metrology Institute of South Africa, 15 Lower Hope Road, Rosebank, 7700 Cape Town, South Africa.

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
|December 17, 2013
PubMed
Summary

The National Metrology Institute of South Africa validated Technetium-99 (Tc-99) radioactivity measurements using three methods. Results confirmed the accuracy of the latest (2011) decay data evaluation for Tc-99.

Keywords:
4π(LS)β–γ coincidence tracingBeta spectral shapeCNETLiquid scintillation countingTDCRTechnetium-99

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Area of Science:

  • Metrology
  • Nuclear Science
  • Radiochemistry

Background:

  • International key comparisons are crucial for harmonizing radioactivity measurements globally.
  • Accurate standardization of pure beta emitters like Technetium-99 (Tc-99) is essential for various applications.

Purpose of the Study:

  • To participate in the international key comparison CCRI(II)-K2.Tc-99 for Tc-99.
  • To validate the standardization of Tc-99 solutions using multiple independent methods.

Main Methods:

  • Utilized three distinct methods for radioactivity standardization: TDCR efficiency calculation, CIEMAT/NIST efficiency tracing, and 4π(LS)β-γ coincidence tracing.
  • Employed Cobalt-60 (Co-60) as a tracer in the coincidence method.
  • Compared results against the latest (2011) Decay Data Evaluation Project (DDEP) evaluation of Tc-99 decay data.

Main Results:

  • Achieved excellent agreement between the results obtained from the three independent standardization methods.
  • Demonstrated consistency in radioactivity measurements for Tc-99.

Conclusions:

  • The study confirms the reliability of the three applied methods for Tc-99 standardization.
  • Validated the applicability of the 2011 DDEP evaluation for Tc-99 beta spectral shape, superseding the 2004 evaluation.