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Updated: May 4, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Novel anthraquinone based chalcone analogues containing an imine fragment: synthesis, cytotoxicity and
Branka Kolundžija1, Violeta Marković2, Tatjana Stanojković1
1Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia.
Abstract:
A new class of imine derivatives of hybrid chalcone analogues containing anthraquinone scaffold was synthesized and evaluated for their in vitro cytotoxic activity against HeLa, LS174, and A549 cancer cells. The compound 5n with furan ring linked to imino group showed potent activity against all target cells with IC50 values ranging from 1.76 to 6.11μM. A mode of action study suggested that compounds induced changes typical for apoptosis in HeLa cells. The most active compounds inhibited tubulogenesis and 5h was found to exhibit a strong anti-angiogenic effect.
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