Related Experiment Video
Updated: May 4, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
[Effectiveness of an educational program for reducing blood culture contamination]
Begoña de Dios García1, Yolanda Lladò Maura1, José Vicente Val-Pérez2
1Unidad Multidisciplinar de Sepsis, Hospital Son Llàtzer, Palma de Mallorca, España.
Introduction:
Blood culture contaminations can lead to unnecessary diagnostic procedures and treatments, increasing workload, length of stay, and costs.
Objetives:
Development of an educational program to reduce contamination rates.
Material And Methods:
Our study compared contamination rates (CR) between a pre-intervention period (Ppre) and post-intervention period (Ppos), where clinical charts from patients with positive blood cultures were reviewed. Intervention consisted of a questionnaire where knowledge of blood culture practice and its significance was assessed. Results are discussed and explained.
Results:
A presentation on blood culture guidelines was discussed in every nurse station. There was a median of 64% (40.8-78.5) attendance rate. The median of correct answers was 69% in the Ppre (54.1-83.3) with 85.7% (83.3-100) in the Ppos, indicating an improvement in 85.7% of the departments that could be compared. There were 136 (4.2%) contaminants in the Ppre and 186 (6.05%) in the Ppos (P=.005). Among the different departments the average of CR varied from 5% vs 7.5% (P=.79) between 2011 and 2012. Only 2 departments reduced CR by 2% to 2.5%, the difference was not significant.
Conclusions:
The intervention failed to reduce overall contamination rates, but knowledge of blood culture practice improved. Our results identified the errors that will help us to design a successful approach in future follow-up programs.
Insights
An educational program improved knowledge of blood culture practices but did not reduce blood culture contamination rates. Future interventions will address identified errors to improve success.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Healthcare Quality Improvement
Background:
- Blood culture contamination leads to increased healthcare costs and unnecessary procedures.
- Reducing contamination is crucial for accurate diagnosis and patient management.
Purpose of the Study:
- To develop and evaluate an educational program aimed at decreasing blood culture contamination rates.
- To assess the impact of the intervention on healthcare professionals' knowledge and practice.
Main Methods:
- A pre- and post-intervention study design was employed.
- Contamination rates (CR) were compared before and after the educational intervention.
- A questionnaire assessed knowledge of blood culture procedures and their significance.
Main Results:
- The educational intervention significantly improved correct answers on the knowledge questionnaire (69% to 85.7%).
- Despite improved knowledge, overall blood culture contamination rates increased from 4.2% to 6.05% (P=.005).
- No significant reduction in contamination rates was observed across most departments.
Conclusions:
- The educational program enhanced knowledge but failed to decrease overall blood culture contamination rates.
- Identified knowledge gaps and errors provide a basis for refining future intervention strategies.
- Further research is needed to develop effective methods for reducing clinical contamination.
More Related Videos
13:39Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Published on: July 7, 2023
10:32Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
Related Concept Videos
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Antimicrobial Effectiveness
Key Techniques in Microbiology
Hand hygiene
Hand washing...
Urine Studies II: Urine Culture and Sensitivity Test
Methods for Controlling Microbial Growth