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Updated: May 4, 2026

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII
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Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry CE-ICP-MS for Quantification of Iron Redox Species FeII, FeIII

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The influence of storage age on iron status, oxidative stress and antioxidant protection in paediatric packed cell

Keith Collard1, Desley White1, Adrian Copplestone2

  • 1University of Plymouth, School of Health Professions, Plymouth, United Kingdom.

Blood Transfusion = Trasfusione Del Sangue
|December 17, 2013
PubMed

Insights

Iron and oxidative damage increase in stored paediatric blood. Antioxidant defenses decline during storage, potentially harming premature infants receiving transfusions.

Area of Science:

  • Biochemistry
  • Neonatal Medicine
  • Blood Transfusion

Background:

  • Blood transfusions in premature infants are linked to bronchopulmonary dysplasia.
  • Iron-induced oxidative damage may contribute to adverse transfusion effects.
  • Storage duration of blood products might influence transfusion outcomes.

Purpose of the Study:

  • To investigate the impact of storage duration on iron and oxidative status in paediatric packed red blood cell units.
  • To assess changes in iron parameters and oxidative damage biomarkers over 35 days of storage.

Main Methods:

  • Paediatric packed red blood cells were sampled at intervals up to 35 days post-donation.
  • Measured extracellular iron (total, binding capacity, non-transferrin-bound), hemoglobin, ascorbate, glutathione, and malondialdehyde.

Main Results:

  • Total and non-transferrin-bound iron increased linearly throughout storage.
  • Hemoglobin and malondialdehyde levels rose, particularly after 21 days.
  • Antioxidants (ascorbate, glutathione) decreased, correlating with increased iron and oxidative damage.

Conclusions:

  • Iron release during storage may stem from oxidative damage to red blood cells.
  • Declining antioxidant capacity exacerbates iron release and further oxidative damage.
  • The use of older stored blood may pose significant risks to infant recipients.
Abstract

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