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Related Experiment Video

Updated: May 4, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
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Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

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An efficient method to isolate and culture mouse Kupffer cells.

Pei-zhi Li1, Jin-zheng Li1, Min Li1

  • 1Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Immunology Letters
|December 17, 2013
PubMed
Summary
This summary is machine-generated.

This study presents an effective, rapid method for isolating pure, functional Kupffer cells (KCs) from mouse liver. The technique yields high numbers of viable KCs, crucial for liver research.

Keywords:
CultureIsolateKupffer cells

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Area of Science:

  • Immunology
  • Hepatology
  • Cell Biology

Background:

  • Kupffer cells (KCs) are vital liver macrophages involved in numerous physiological and pathological processes.
  • Existing KC isolation methods are often complex, time-consuming, and primarily optimized for human or rat livers.

Purpose of the Study:

  • To develop and validate an efficient, high-yield method for isolating pure Kupffer cells from mouse liver.
  • To ensure the isolated KCs retain their characteristic morphology and functional integrity.

Main Methods:

  • A three-step isolation procedure involving enzymatic tissue digestion, gradient centrifugation, and selective adherence.
  • F4/80 immunofluorescence and flow cytometry for cell identification and purity assessment.
  • Assessment of cell viability, morphology, phagocytic activity, and TNF-α production post-isolation.

Main Results:

  • The method successfully isolated 5-6×10^6 Kupffer cells per mouse liver with >92.0% F4/80 purity and 98.5% viability.
  • Isolated KCs exhibited typical macrophage morphology, strong phagocytic activity, and maintained functional capacity, including LPS-induced TNF-α production.
  • The procedure is less time-consuming and uses less collagenase compared to traditional methods.

Conclusions:

  • This optimized three-step method provides an effective means to obtain high-purity, high-yield, and functionally intact Kupffer cells from mouse liver.
  • The technique is suitable for various research applications requiring reliable mouse KC isolation.
  • This simplified approach facilitates further investigation into liver immunology and disease.