Related Experiment Video
Updated: May 4, 2026
08:05
Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation
Published on: July 3, 2017
11.6K
Acridine orange leukocyte fluorography in mice
Judd M Cahoon1, Paul R Olson1, Spencer Nielson1
1Department of Ophthalmology and Visual Science, John A Moran Eye Center, University of Utah, Salt Lake City, UT 84109, United States.
Experimental Eye Research
|December 17, 2013
Summary
This study presents a standardized method for visualizing leukocyte behavior in the mouse retina using acridine orange and a scanning laser ophthalmoscope. This technique aids in understanding retinopathies and inflammation research.
Area of Science:
- Ophthalmology
- Immunology
- Biomedical Imaging
Background:
- Retinal vasculature is crucial for understanding retinopathies.
- Leukocyte adhesion and inflammation are key in many retinopathies.
- Previous leukocyte fluorography methods lacked standardization.
Purpose of the Study:
- To establish a standardized, convenient method for visualizing leukocyte behavior in the mouse retina.
- To enable non-invasive, simultaneous visualization of retinal vasculature and nerves.
- To facilitate research on leukocyte transit, adherence, and extravasation in retinopathies.
Main Methods:
- Utilized acridine orange (AO) as a fluorescent marker.
- Employed a commercially available scanning laser ophthalmoscope (SLO, HRA-OCT Spectralis).
- Compared leukocyte behavior in normal mice versus those with acute and chronic inflammation.
Main Results:
- Successfully visualized leukocyte behavior in the mouse retina.
- Demonstrated differences in leukocyte activity between normal and inflamed retinas.
- Provided a detailed protocol for reproducible leukocyte fluorography.
Conclusions:
- The described method offers a standardized approach for studying retinal inflammation.
- This technique can be readily adopted by research laboratories.
- Facilitates deeper understanding of leukocyte dynamics in retinopathies.
