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Updated: May 4, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
On-line sample preconcentration by large-volume sample stacking with an electroosmotic flow pump (LVSEP) in
Fumihiko Kitagawa1, Takayuki Kawai, Koji Otsuka
1Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University.
Abstract:
This review highlights on-line sample preconcentration by large-volume sample stacking with an electroosmotic flow pump (LVSEP) in microscale electrophoresis. LVSEP requires only a hydrodynamic sample injection into the whole capillary/microchannel and a constant voltage application, i.e., without polarity switching, providing an efficient sensitivity enhancement with a relatively easy experimental procedure without loss of the separation efficiencies. Recent developments and applications of LVSEP are briefly reviewed from the first LVSEP study in 1999 with categorization on the basis of separation modes, including zone electrophoresis, cyclodextrin- and affinity-based separation modes and non-aqueous capillary electrophoresis.
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