Locomotion of microspheres for super-resolution imaging

Super-resolution virtual imaging by micron sized transparent beads (microspheres) was recently demonstrated by Wang et al. Practical applications in microscopy require control over the positioning of the microspheres. Here we present a method of positioning and controllable movement of a microsphere by using a fine glass micropipette. This allows sub-diffraction imaging at arbitrary points in three dimensions, as well as the ability to track moving objects. The results are relevant to a broad scope of applications, including sample inspection, microfabrication, and bio-imaging.