Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fabrication of Nd<sup>3+</sup> and Yb<sup>3+</sup> doped NIR emitting nano fluorescent probe: A candidate for bioimaging applications.

Materials science & engineering. C, Materials for biological applications·2021

Same author

NaGdF<sub>4</sub>:Yb,Er-Ag nanowire hybrid nanocomposite for multifunctional upconversion emission, optical imaging, MRI and CT imaging applications.

Mikrochimica acta·2020

Same author

Association analysis of the major histocompatibility complex region in psoriasis vulgaris.

The British journal of dermatology·2019

Same author

Revealing Color Forces with Transverse Polarized Electron Scattering.

Physical review letters·2019

Same author

First Report of Nigrospora Leaf Blight on Sesame Caused by Nigrospora sphaerica in China.

Plant disease·2019

Same author

Identification and Genetic Diversity of Formae Speciales of Setosphaeria turcica in China.

Plant disease·2019

Search research articles

Related Experiment Video

Updated: May 4, 2026

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Published on: June 18, 2014

Stokes emission in GdF₃:Nd³⁺ nanoparticles for bioimaging probes.

M Pokhrel¹, L C Mimun, B Yust

¹Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas 78249, USA. ekf012@my.utsa.edu.

|December 17, 2013

Summary

Rare earth doped nanoparticles exhibit efficient near-infrared photoluminescence, making them ideal for deep-tissue biological imaging. GdF₃:Nd(3+) nanoparticles show significantly higher quantum yields than traditional upconversion particles.

More Related Videos

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Related Experiment Videos

Last Updated: May 4, 2026

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Published on: June 18, 2014

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Area of Science:

Materials Science
Nanotechnology
Biomedical Imaging

Background:

Rare earth (RE) doped nanoparticles (NPs) are of interest for near-infrared (NIR) photoluminescence (PL).
NIR-based NPs offer deep-tissue bioimaging with high contrast and resolution due to minimal autofluorescence.

Purpose of the Study:

To investigate highly efficient infrared photoluminescence in Gadolinium Fluoride:Neodymium (GdF₃:Nd(3+)) nanoparticles.
To evaluate the downconversion quantum yields (QY) of these nanoparticles in various forms (powder, coated, colloidal).
To demonstrate their utility as probes for bioimaging applications.

Main Methods:

Synthesis of GdF₃:Nd(3+) nanoparticles.
Characterization of hydrodynamic size for bio-applications.
Measurement of absolute quantum yields (QY) for downconversion (Stokes emission).
Comparison with established upconversion (UC) particles (NaYF₄:Er/Yb).
Confocal imaging and spectroscopic studies for bioimaging demonstration.

Main Results:

GdF₃:Nd(3+) nanoparticles exhibit highly efficient infrared photoluminescence under 800 nm excitation.
Colloidal GdF₃:1% Nd(3+) NPs (approx. 5 ± 2 nm) demonstrated downconversion QYs 2000 times higher than efficient UC particles.
Successful demonstration of NIR emitting nanoparticles as probes via confocal imaging and spectroscopy.

Conclusions:

GdF₃:Nd(3+) nanoparticles are highly efficient NIR emitters suitable for bioimaging.
Their superior downconversion quantum yields offer significant advantages over existing UC nanoparticles.
These NPs show great promise as advanced probes for in vivo bioimaging applications.