Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adjunctive Normobaric Hyperoxia With Endovascular Thrombectomy for Acute Stroke at 6 to 24 Hours: A Phase IIb Randomized Trial.

Stroke·2026
Same author

Red-Emissive Copper-Gold Bimetallic Metal-Organic Gel Nanozyme as a Highly Sensitive and Selective Ratiometric Photoluminescent Probe for Cellular Thiamine Detection.

Analytical chemistry·2026
Same author

Trityl-Nitroxide Triradicals for Efficient High-Field Dynamic Nuclear Polarization.

Analytical chemistry·2026
Same author

Photocrosslinked polylysine/oxidized hyaluronic acid double-network hydrogel with anti-inflammatory and antibacterial functions for skin wound healing.

International journal of biological macromolecules·2026
Same author

Nanodelivery of Gentiopicroside for Inflammatory Skin Lesions: Insights from Psoriasis and Diabetic Foot Ulcers.

International journal of nanomedicine·2026
Same author

Association of triglyceride-glucose index combined with Chinese visceral adiposity index and cardiovascular diseases in middle-aged and older adults: a cohort study.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2026

Related Experiment Video

Updated: May 4, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

18.0K

Highly sensitive free radical detection by nitrone-functionalized gold nanoparticles.

Libo Du1, Saipeng Huang, Qianfen Zhuang

  • 1State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Centre for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. China. yliu@iccas.ac.cn.

Nanoscale
|December 17, 2013
PubMed
Summary

Researchers developed novel gold nanoparticles functionalized with nitrone (Au@EMPO) for highly efficient free radical detection. This new nanospin trap significantly enhances sensitivity and reaction rates for hydroxyl radicals (˙OH) in biological systems.

More Related Videos

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.4K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K

Related Experiment Videos

Last Updated: May 4, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

18.0K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.4K
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

7.7K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Free radicals play crucial roles in physiological and pathological processes.
  • Electron spin resonance (ESR) with spin trapping is specific for radical detection but limited in biological applications due to slow reaction rates.
  • Current spin traps exhibit low reaction rates with biological radicals, hindering in vivo applications.

Purpose of the Study:

  • To develop highly efficient spin traps for detecting free radicals in biological systems.
  • To overcome the limitations of low reaction rates in existing spin trapping techniques.
  • To introduce a novel nanospin trap with enhanced properties for radical detection.

Main Methods:

  • Self-assembly of a thiolated nitrone derivative (EMPO) onto gold nanoparticles to create Au@EMPO.
  • Kinetic studies to compare the reaction rate constants of Au@EMPO with existing spin traps like PBN.
  • Evaluation of the stability and sensitivity of the Au@EMPO spin adduct for hydroxyl radical (˙OH) detection.

Main Results:

  • Au@EMPO demonstrated a 137-fold higher reaction rate constant with hydroxyl radicals (˙OH) compared to PBN.
  • The resulting Au@EMPO spin adduct exhibited high stability with a half-life of approximately 56 minutes.
  • Au@EMPO provided 124-fold higher sensitivity for ˙OH detection compared to the original EMPO spin trap.

Conclusions:

  • Au@EMPO represents a novel and highly efficient nanospin trap for detecting free radicals, particularly ˙OH.
  • The enhanced reaction rate and stability of Au@EMPO significantly improve sensitivity for radical detection in biological systems.
  • This work offers a new strategy for designing advanced spin traps with superior performance for various applications.