Related Experiment Video

Updated: May 4, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.5K

RETRACTED: miRNA-302b suppresses human hepatocellular carcinoma by targeting AKT2

Lumin Wang1, Jiayi Yao, Xiaogang Zhang

  • 1Department of Genetics and Molecular Biology, Xi'an Jiaotong, University School of Medicine, 76 Yan Ta West Road, Xi'an, Shaanxi 710061, China. hchen@mail.xjtu.edu.cn.

Molecular Cancer Research : MCR
|December 17, 2013
PubMed
Abstract

Insights

MicroRNA-302b (miR-302b) acts as a tumor suppressor in hepatocellular carcinoma (HCC). This study found miR-302b inhibits HCC cell proliferation and growth by targeting AKT2.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial in human cancers.
  • The role of miR-302b in hepatocellular carcinoma (HCC) was previously unknown.
  • miR-302b is a potential tumor suppressor in various cancers.

Purpose of the Study:

  • To investigate the expression and function of miR-302b in HCC.
  • To identify the molecular targets of miR-302b in HCC.
  • To determine the therapeutic potential of miR-302b in HCC.

Main Methods:

  • Quantitative real-time PCR to assess miR-302b expression.
  • In vitro cell proliferation and cell cycle assays.
  • Luciferase reporter assays to identify direct targets.
  • Western blotting to analyze protein expression.
  • In vivo tumor xenograft models.

Main Results:

  • miR-302b expression was significantly downregulated in HCC tissues and cell lines.
  • Overexpression of miR-302b suppressed HCC cell proliferation and G1-S transition.
  • AKT2 was identified as a direct target of miR-302b.
  • Silencing AKT2 mimicked the tumor-suppressive effects of miR-302b.
  • miR-302b repressed HCC tumor growth in vivo.

Conclusions:

  • miR-302b functions as a tumor suppressor in hepatocellular carcinoma.
  • miR-302b inhibits HCC cell proliferation and growth by targeting AKT2.
  • miR-302b holds potential as a therapeutic agent for HCC.

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.1K
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

38.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
MicroRNAs01:22

MicroRNAs

9.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K