A solid-phase fluorescent biosensor for the determination of phenolic compounds and peroxides in samples with complex...

Area of Science:

• Analytical Chemistry
• Biochemistry
• Materials Science

Background:

• Traditional methods for detecting phenolic compounds and peroxides often require complex sample pretreatment.
• Existing biosensors may struggle with complex matrices like non-transparent or water-insoluble samples.
• There is a need for rapid, sensitive, and robust analytical tools for these compounds.

Purpose of the Study:

• To develop a simple, solid-phase fluorescent biosensor for determining phenolic compounds and peroxides.
• To enable direct analysis of complex and challenging sample matrices without prior preparation.
• To establish novel fluorescent indicator reactions for broad concentration range detection.

Main Methods:

• Development of a solid-phase biosensor utilizing a peroxidase-chitosan sensitive layer.
• Direct measurement of the analytical signal on the sensor's biosensitive layer.
• Implementation of two novel fluorescent indicator reactions for analyte quantification.

Main Results:

• The developed biosensor demonstrated a simple construction and direct signal measurement.
• Successful analysis of complex matrices, including water-insoluble and non-transparent samples, was achieved without pretreatment.
• The sensor accurately determined phenolic compounds and peroxides across a wide concentration range (nmol L⁻¹ to mmol L⁻¹).

Conclusions:

• The solid-phase fluorescent biosensor is effective for the determination of phenolic compounds and peroxides.
• The sensor's ability to analyze complex samples directly simplifies analytical procedures.
• The developed method shows promise for applications in analyzing various real-world samples like urine, cosmetics, and pharmaceuticals.