Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

1.3K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

METTL3-catalyzed m6A modification of NSUN4 mediates m5C modification of SLC2A3 mRNA to regulate PDGF-BB-induced proliferation, hyaluronan production and glycolysis in human orbital fibroblasts.

Journal of bioenergetics and biomembranes·2026
Same author

Global crop introduction drives host jumps, turning native pathogens into emerging diseases.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Powdery mildews on <i>Fragaria</i> spp. and <i>Rubus</i> spp. - unravelling the phylogeny and taxonomy of economically relevant species within the intricate <i>Podosphaera aphanis</i> s. lat. complex.

Mycoscience·2026
Same author

Revision of powdery mildews (<i>Ascomycota</i>, <i>Erysiphaceae</i>) on <i>Rosa</i> in China: unexpected taxonomic complexity with phytopathological implications.

IMA fungus·2026
Same author

Discovery of two new <i>Phaeoclavulina</i> species (Basidiomycota, Gomphaceae) from high-elevation habitats in the Qinghai-Xizang Plateau.

MycoKeys·2026
Same author

A novel amide-quinoline salt-based mitochondrial-targeted fluorescent probe for detecting pH in cells and water samples.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Related Experiment Video

Updated: May 4, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

6.6K

Novel pyrazoline-based selective fluorescent sensor for Hg2+.

Sheng-Qing Wang1, Shu-Yan Liu, Hao-Yan Wang

  • 1School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People's Republic of China.

Journal of Fluorescence
|December 17, 2013
PubMed
Summary

A novel pyrazoline compound acts as a highly sensitive fluorescent sensor for detecting mercury ions (Hg2+). This sensor demonstrates excellent selectivity and has potential applications in cellular imaging.

More Related Videos

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

4.9K

Related Experiment Videos

Last Updated: May 4, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII
05:15

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

6.6K
IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

4.9K

Area of Science:

  • Chemical Sensing
  • Fluorescence Spectroscopy
  • Materials Science

Background:

  • Development of selective and sensitive chemosensors is crucial for environmental monitoring and biological studies.
  • Mercury ions (Hg2+) pose significant environmental and health risks, necessitating accurate detection methods.

Purpose of the Study:

  • To synthesize a pyrazoline derivative with specific optical properties.
  • To evaluate the compound's efficacy as a fluorescent sensor for mercury ions (Hg2+).
  • To explore its potential for practical applications, including cellular imaging.

Main Methods:

  • Synthesis and characterization of a pyrazoline compound.
  • Investigation of UV-Vis absorption and fluorescence emission properties.
  • Testing selectivity and sensitivity towards Hg(2+) in a mixed solvent system (EtOH:H2O).
  • Determination of the sensor's complexation ratio and detection limit.
  • In vitro cell imaging experiments.

Main Results:

  • The synthesized pyrazoline compound exhibits distinct UV-Vis absorption and fluorescence emission characteristics.
  • The compound selectively and sensitively detects Hg(2+) ions, forming a 1:1 complex.
  • A significant fluorescent enhancement was observed upon Hg(2+) binding, with good tolerance to other metal ions.
  • A low fluorometric detection limit of 3.85 × 10(-10) M for Hg(2+) was achieved.
  • The fluorescent probe demonstrated successful application in live cell imaging.

Conclusions:

  • The developed pyrazoline-based sensor offers a highly sensitive and selective method for Hg(2+) detection.
  • The sensor's ability to function in a mixed aqueous-organic medium and its cell imaging capability highlight its practical utility.
  • This research provides a promising tool for environmental monitoring and biological sensing of mercury.