Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A protein binding assay for hyaluronate.

B E Lacy, C B Underhill

    Analytical Biochemistry
    |November 1, 1986
    PubMed
    Summary

    A new assay using hyaluronectin, a specific binding protein, accurately quantifies hyaluronate. This method is sensitive and unaffected by proteins, offering a reliable way to measure hyaluronate levels.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Major Trends in Gastroenterology and Hepatology Between 2010 and 2019: An Overview of Advances From the Past Decade Selected by the Editorial Board of The American Journal of Gastroenterology.

    The American journal of gastroenterology·2020
    Same author

    Effects of Antidepressants on Gastric Function in Patients with Functional Dyspepsia.

    The American journal of gastroenterology·2017
    Same author

    Emerging treatments in neurogastroenterology: eluxadoline - a new therapeutic option for diarrhea-predominant IBS.

    Neurogastroenterology and motility·2015
    Same author

    The impact of obesity on oesophageal acid exposure time on and off proton pump inhibitor therapy.

    Alimentary pharmacology & therapeutics·2015
    Same author

    Treatment for constipation: new and old pharmacological strategies.

    Neurogastroenterology and motility·2014
    Same author

    Responders vs clinical response: a critical analysis of data from linaclotide phase 3 clinical trials in IBS-C.

    Neurogastroenterology and motility·2014

    Area of Science:

    • Biochemistry
    • Analytical Chemistry

    Background:

    • Hyaluronate is a crucial glycosaminoglycan with diverse biological roles.
    • Accurate quantification of hyaluronate is essential for various research and diagnostic applications.

    Purpose of the Study:

    • To develop a rapid and straightforward assay for quantifying hyaluronate.
    • To utilize the specific binding protein, hyaluronectin, for hyaluronate detection.

    Main Methods:

    • Hyaluronectin was isolated from rat brain homogenates.
    • A binding assay was established using radiolabeled hyaluronate ([3H]hyaluronate) and hyaluronectin.
    • Quantification was achieved through a competition assay with unlabeled hyaluronate.

    Main Results:

    • Hyaluronectin demonstrated specific binding to hyaluronate, with other glycosaminoglycans showing no competition.
    • The binding exhibited high affinity (Kd = 5.7 X 10(-10) M).
    • The assay is sensitive to 1 microgram or less of hyaluronate and is protein-independent.

    Conclusions:

    • A robust and sensitive assay for hyaluronate quantification has been successfully developed.
    • The assay's specificity and sensitivity make it valuable for biological and clinical research.
    • Hyaluronectin serves as an effective binding protein for hyaluronate detection.

    Related Experiment Videos