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Related Experiment Videos

Studies on myelin-basic-protein methylation during mouse brain development.

L P Chanderkar, W K Paik, S Kim

    The Biochemical Journal
    |December 1, 1986
    PubMed
    Summary

    Myelin basic protein (MBP) synthesis and methylation were studied in developing mouse brains. Younger brains showed higher methylation relative to synthesis, correlating with increased protein methylase I activity.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Developmental Biology

    Background:

    • Myelin basic protein (MBP) is crucial for myelin sheath formation in the central nervous system.
    • Understanding the regulation of MBP synthesis and post-translational modifications like methylation is key to comprehending brain development.

    Purpose of the Study:

    • To investigate the in vivo synthesis and methylation of MBP during mouse brain development.
    • To correlate MBP methylation patterns with protein methylase I (PMI) activity and MBP species composition.

    Main Methods:

    • Intracerebral injection of radiolabeled methionine (L-[methyl-3H]methionine and L-[35S]methionine) in mice of varying ages (13-60 days).
    • Isolation and quantification of radioactivity in MBP.
    • Determination of the 3H/35S ratio (methylation index) in MBP fractions.

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  • Analysis of MBP species by SDS/polyacrylamide-gel electrophoresis.
  • Assay of protein methylase I (PMI) activity.

    • Main Results:

    • Radioactivity incorporation into MBP was highest in the youngest brains and decreased with age, inversely correlating with total MBP levels.
    • The methylation index (3H/35S ratio) was highest in the youngest brains and in isolated MBP fractions, correlating positively with PMI activity.
    • MBP species separated by size showed developmental changes, with high-molecular-weight MBP predominant in younger brains and smaller MBP in older brains.

    Conclusions:

    • MBP methylation is more prominent relative to synthesis in younger developing mouse brains.
    • Protein methylase I activity plays a significant role in the developmental methylation patterns of MBP.
    • The observed developmental shifts in MBP species and their methylation status are important for understanding myelin maturation.