Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monoclonal antibody-recognizing cone visual pigment.

A Szél, L Takács, E Monostori

    Experimental Eye Research
    |December 1, 1986
    PubMed
    Summary

    Researchers developed monoclonal antibodies to study chicken retina photoreceptors. Antibody COS-1 specifically targets double cones and one single cone type, identifying a 33,000 MW protein, likely a visual pigment.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The effects of prenatal, perinatal and neonatal factors on academic performance in primary school age children.

    Ceska gynekologie·2020
    Same author

    Psychosocial risk factors for emergency cesarean section.

    Ceska gynekologie·2019
    Same author

    [Are womens attitudes towards pregnancy, childbirth and motherhood associated with length of labour?]

    Ceska gynekologie·2018
    Same author

    Systemic lupus erythematosus in the light of the regulatory effects of galectin-1 on T-cell function.

    Lupus·2017
    Same author

    [Postpartum blues - a Czech adaptation of the Maternity Blues Questionnaire].

    Ceska gynekologie·2016
    Same author

    [Satisfaction with perinatal care in Vysočina region in the period between October 2013 and September 2014].

    Ceska gynekologie·2016

    Area of Science:

    • Ophthalmology
    • Immunology
    • Molecular Biology

    Background:

    • Photoreceptor membranes contain crucial visual pigments.
    • Chicken retinas possess diverse photoreceptor types, including rods and cones.
    • Monoclonal antibodies are valuable tools for precise molecular identification.

    Purpose of the Study:

    • To generate and characterize monoclonal antibodies against chicken retinal photoreceptor proteins.
    • To identify specific proteins within cone outer segments using immunocytochemistry and immunoblotting.
    • To investigate the distribution and molecular identity of visual pigments in chicken retinas.

    Main Methods:

    • Raising monoclonal antibodies against chicken retinal photoreceptor membranes.
    • Screening antibody clones using immunocytochemistry on retinal sections.

    Related Experiment Videos

  • Analyzing antibody binding to photoreceptor proteins via immunoblotting.
  • Characterizing protein molecular masses and identifying visual pigments using specific antibodies.

    • Main Results:

    • Monoclonal antibody COS-1 selectively labeled outer segments of double cones and one type of single cone.
    • COS-1 recognized a 33,000 MW protein in photoreceptor membranes.
    • Monoclonal antibody OS-2 labeled all outer segments and recognized multiple proteins, including rhodopsin, suggesting it targets a common visual pigment epitope.
    • COS-1 demonstrated cross-reactivity with cone outer segments in various vertebrate species.

    Conclusions:

    • Monoclonal antibody COS-1 is a specific marker for certain cone types in the chicken retina.
    • The 33,000 MW protein recognized by COS-1 is likely a cone visual pigment.
    • Monoclonal antibody OS-2 targets a conserved epitope across chicken visual pigments.
    • These antibodies provide valuable tools for studying photoreceptor structure and function across vertebrates.