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Updated: May 4, 2026
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
Published on: February 24, 2017
BMP-9 induces rabbit adipose-derived stem cells to differentiation into osteoblasts via BMP signaling pathway
Yi Chai1, Fajing Liu1, Qian Li1
1Department of Spine Surgery, Third Hospital of HeBei Medical University, Shijiazhuang, China.
Objective:
To explore the role of BMP-9 in osteoblast differentiation from adipose-derived stem cells (ADSCs).
Study Design:
Rabbit ADSCs were isolated from subcutaneous tissues of the abdomen and inguinal fat pads and then purified and expanded in vitro. AdBMP9, SB203580 (P38 MAPK inhibitors), and PD98059 (ERK1/2 inhibitor) were used for osteoblastic differentiation.
Results:
The results show that bone morphogenetic proteins (BMPs) could affect the differential direction of ADSCs. Polymerase chain reaction assays reveal the important role of BMP signaling pathway in osteoblastic differentiation of ADSCs, and the members included Smad 1, Smad 4, Smad 5, Smad 8, P38, ERK1/2, Runx 2, collagen type I and osteopontin.
Conclusion:
This study provides some theoretical basis and experimental evidence for the application of ADSCs into treatment of bone injury.
