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Updated: May 4, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Signal transduction molecule patterns indicating potential glioblastoma therapy approaches
Maria Linda Cruceru1, Ana-Maria Enciu2, Adrian Claudiu Popa3
1Carol Davila University of Medicine and Pharmacy, Department of Cellular and Molecular Medicine, Bucharest, Romania.
Purpose:
The expression of an array of signaling molecules, along with the assessment of real-time cell proliferation, has been performed in U87 glioma cell line and in patients' glioblastoma established cell cultures in order to provide a better understanding of cellular and molecular events involved in glioblastoma pathogenesis. Experimental therapy was performed using a phosphatidylinositol-3'-kinase (PI3K) inhibitor.
Patients And Methods:
xMAP technology was employed to assess expression levels of several signal transduction molecules and real-time xCELLigence platform for cell behavior.
Results:
PI3K inhibition induced the most significant effects on global signaling pathways in patient-derived cell cultures, especially on members of the mitogen-activated protein-kinase family, P70S6 serine-threonine kinase, and cAMP response element-binding protein expression and further prevented tumor cell proliferation.
Conclusion:
The PI3K pathway might be a prime target for glioblastoma treatment.
Insights
Targeting the phosphatidylinositol-3'-kinase (PI3K) pathway significantly impacts glioblastoma cell signaling and proliferation. PI3K inhibition shows promise as a therapeutic strategy for glioblastoma treatment.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cellular Signaling
Background:
- Glioblastoma pathogenesis involves complex cellular and molecular events.
- Understanding signaling pathways is crucial for developing effective glioblastoma therapies.
Purpose of the Study:
- To investigate signaling molecule expression and cell proliferation in glioblastoma.
- To evaluate the therapeutic potential of targeting the phosphatidylinositol-3 '-kinase (PI3K) pathway.
Main Methods:
- Utilized xMAP technology to measure signal transduction molecule expression.
- Employed the xCELLigence platform for real-time assessment of glioblastoma cell behavior.
- Applied a PI3K inhibitor in experimental therapy.
Main Results:
- PI3K inhibition profoundly affected global signaling pathways in patient-derived glioblastoma cultures.
- Key affected pathways included mitogen-activated protein-kinase family, P70S6 serine-threonine kinase, and cAMP response element-binding protein.
- PI3K inhibition effectively suppressed glioblastoma tumor cell proliferation.
Conclusions:
- The PI3K pathway represents a significant therapeutic target for glioblastoma.
- Targeting PI3K may offer a viable strategy for glioblastoma treatment.
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