Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and Immunogenicity of a Nanoparticle Combination Vaccine for Salmonella in Poultry.

Plant biotechnology journal·2026
Same author

Investigating substrate specificity in arogenate versus prephenate dehydratases.

BMC biology·2026
Same author

Simultaneous modification of Thermotoga maritima encapsulin subunits to produce multivalent nanoparticle vaccines for non-typhoidal Salmonella enterica.

The FEBS journal·2026
Same author

Protein nanoparticle-based vaccine candidate produced in <i>Nicotiana benthamiana</i> against non-typhoidal <i>Salmonella enterica</i> in poultry.

Frontiers in plant science·2026
Same author

Cell-type-specific immune programs orchestrate spatial defense in the Arabidopsis leaf epidermis.

Nature communications·2026
Same author

NUCLEOPORIN1 mediates proteasome-based degradation of ABI5 to regulate Arabidopsis seedling establishment.

The Plant journal : for cell and molecular biology·2026

Related Experiment Video

Updated: May 4, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

11.0K

Reprogramming cells to study vacuolar development.

Mistianne Feeney1, Lorenzo Frigerio2, Susanne E Kohalmi3

  • 1Department of Biology, University of Western Ontario London, ON, Canada ; Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada London, ON, Canada ; School of Life Sciences, University of Warwick Coventry, UK.

Frontiers in Plant Science
|December 19, 2013
PubMed
Summary

Cellular reprogramming in Arabidopsis thaliana successfully converted lytic vacuoles (LVs) to protein storage vacuoles (PSVs) in vegetative cells. This method offers a new way to study plant cell fate transitions during development.

Keywords:
Arabidopsis thalianaLEAFY COTYLEDON2cellular reprogrammingdevelopmental transitionlytic vacuoleprotein storage vacuolevacuole biogenesis

More Related Videos

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

8.9K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

2.9K

Related Experiment Videos

Last Updated: May 4, 2026

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

11.0K
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

8.9K
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
05:04

In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter

Published on: February 22, 2022

2.9K

Area of Science:

  • Plant Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Plant cells undergo significant reorganization during developmental transitions.
  • Studying these changes in Arabidopsis thaliana embryos is challenging.
  • Vacuoles in plants, such as lytic vacuoles (LVs) and protein storage vacuoles (PSVs), change function and morphology during development.

Purpose of the Study:

  • To investigate the transition mechanism between LVs and PSVs.
  • To demonstrate cellular reprogramming as a tool for studying developmental transitions.
  • To explore the role of LEAFY COTYLEDON2 in cell fate changes.

Main Methods:

  • Overexpression of LEAFY COTYLEDON2 in Arabidopsis thaliana vegetative cells.
  • Genetic manipulation to activate the seed developmental program.
  • Microscopic observation of vacuole morphology and function.

Main Results:

  • Vegetative cells successfully reprogrammed to exhibit a seed development program.
  • Arabidopsis leaf LVs were observed to convert into PSV-like organelles.
  • This study provides a model system for studying LV to PSV transitions.

Conclusions:

  • Cellular reprogramming is a viable method to study challenging developmental processes in plants.
  • The study elucidates a potential mechanism for LV to PSV transitions.
  • Further research can build upon this model to understand plant cell fate determination.