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Updated: May 4, 2026

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
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Nucleosome-binding protein HMGN2 exhibits antitumor activity in oral squamous cell carcinoma

Ankang Hu1, Xiaoqian Dong1, Xiqian Liu1

  • 1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Oncology Letters
|December 19, 2013
PubMed

Insights

High mobility group nucleosomal binding domain 2 (HMGN2) protein inhibits oral squamous cell carcinoma growth by inducing apoptosis. This suggests HMGN2 may act as an antitumor effector molecule for natural killer (NK) and cytolytic T lymphocytes (CTLs).

Area of Science:

  • Immunology
  • Molecular Biology
  • Oncology

Background:

  • Natural killer (NK) cells and cytolytic T lymphocytes (CTLs) are crucial for antitumor immune responses.
  • High mobility group nucleosomal binding domain 2 (HMGN2) is implicated as a potential effector molecule in NK and CTL functions.

Purpose of the Study:

  • To investigate the antitumor effects of recombinant human HMGN2 on oral squamous cell carcinoma (OSCC).
  • To explore the underlying molecular mechanisms of HMGN2-induced growth inhibition and apoptosis in OSCC cells.

Main Methods:

  • Recombinant human HMGN2 was purified from transformed Escherichia coli.
  • Tca8113 OSCC cells were treated with varying concentrations of HMGN2.
  • Cell growth, apoptosis rates, and protein expression (p53, Bax, Bcl-2, caspase-3) were analyzed using cell-based assays and Western blotting.

Main Results:

  • HMGN2 significantly inhibited Tca8113 cell growth and induced S-phase arrest.
  • HMGN2 treatment led to increased apoptosis, evidenced by elevated p53 and Bax, decreased Bcl-2, and activated caspase-3.
  • Preliminary data suggest potential in vivo suppression of Tca8113 cell growth by HMGN2.

Conclusions:

  • HMGN2 protein demonstrates significant inhibitory effects on oral squamous cell carcinoma growth.
  • HMGN2 may function as a novel antitumor effector molecule for NK cells and CTLs in OSCC treatment.
Keywords:
HMGN2antitumor activityapoptosiscell cycleoral squamous cell carcinoma

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