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Updated: May 4, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Recent progress in tissue optical clearing.

Dan Zhu¹, Kirill V Larin², Qingming Luo¹

¹Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology Wuhan, China ; Key Laboratory of Biomedical Photonics of Ministry of Education, Department of Biomedical Engineering, Huazhong University of Science and Technology Wuhan, China.

Laser & Photonics Reviews

|December 19, 2013

Summary

This summary is machine-generated.

Tissue optical clearing enhances advanced optical methods in life sciences by improving agent penetration and enabling high-resolution 3D imaging of tissues like the brain. This technique also aids in detecting pathological conditions.

Keywords:

OCT blood-flow imaging microscopy imaging molecular diffusion optical imaging optical-clearing agents scattering tissue

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Last Updated: May 4, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

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Published on: June 30, 2016

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

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Published on: July 11, 2025

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

Area of Science:

Biomedical Optics
Life Sciences
Histology

Background:

Advanced optical methods are crucial for life sciences research.
Tissue properties often limit optical penetration and imaging resolution.
Optical clearing offers a promising solution to overcome these limitations.

Purpose of the Study:

To review recent developments in tissue optical clearing techniques.
To discuss the mechanisms, methods, and applications of tissue optical clearing.
To highlight the potential of optical clearing in diagnostics and therapy.

Main Methods:

Overview of physical, molecular, and physiological mechanisms of optical clearing.
Introduction of methods to enhance optical clearing agent penetration (physical, chemical, combined).
Integration of optical clearing with advanced microscopy and labeling techniques.

Main Results:

Demonstration of high-resolution 3D imaging of whole tissues (e.g., brain).
Identification of optical clearing agent diffusion differences in healthy vs. pathological tissues as a diagnostic indicator.
Introduction of advances in in vivo imaging and phototherapy using optical clearing.

Conclusions:

Tissue optical clearing significantly advances the application of optical methods in life sciences.
The technique enables unprecedented resolution for 3D tissue microstructure analysis.
Optical clearing holds potential for disease diagnostics and therapeutic applications.