Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma
Deepika Kanojia1, Manoj Garg1, Shikha Saini1
1Cancer Microarray, Genes and Proteins Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India.
Plos One
|December 19, 2013
Summary
Sperm associated antigen 9 (SPAG9) is expressed in most bladder cancers and may be a biomarker for early-stage disease. Inhibiting SPAG9 reduced cancer cell growth and spread, suggesting it as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Urology
Background:
- Transitional cell carcinoma (TCC) is the most common and chemoresistant bladder cancer.
- Sperm associated antigen 9 (SPAG9) role in bladder TCC requires investigation.
Purpose of the Study:
- To analyze SPAG9 expression and its association with clinical parameters in bladder TCC.
- To investigate the functional role of SPAG9 in bladder cancer cell lines.
Main Methods:
- SPAG9 expression and humoral response assessed in 125 TCC patients and 4 cell lines.
- SPAG9 gene silencing used to evaluate effects on proliferation, cell cycle, migration, and invasion in UM-UC-3 cells.
Main Results:
- SPAG9 expressed in 81% of TCC tissues and all cell lines.
- High SPAG9 expression linked to superficial, low-grade tumors (P=.042, P=.002).
- SPAG9 silencing induced G0-G1 arrest and reduced cancer cell growth, migration, and invasion.
Conclusions:
- SPAG9 is a potential biomarker for bladder TCC.
- SPAG9 represents a promising therapeutic target for bladder TCC.
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