Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Overview01:13

Mass Analyzers: Overview

2.0K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
2.0K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.6K
Uncertainty in Measurement: Accuracy and Precision03:37

Uncertainty in Measurement: Accuracy and Precision

93.9K
Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value. 
93.9K
Accuracy and Precision01:52

Accuracy and Precision

12.5K
Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.  Highly accurate...
12.5K
Accuracy and Precision01:52

Accuracy and Precision

2.9K
2.9K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

993
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
993

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring Molecular Dynamics and Structure Change of Dissolved Organic Nitrogen under Nitrate and Phosphate Pulses in a Cascade Upflow Biofiltration System.

Environmental science & technology·2026
Same author

Proximity Labelling Reveals the Compartmental Proteome of Murine Sensory Neurons.

European journal of pain (London, England)·2026
Same author

Enhanced CT‑based radiomics model to predict CD40LG expression and clinical prognosis in head and neck squamous cell carcinoma.

Brazilian journal of otorhinolaryngology·2026
Same author

Proteome-assisted multi-feature discriminant modelling of chronic post-surgical pain in female patients: A proof-of-concept study.

Pharmacological research·2026
Same author

Progressive neurodegeneration in human dorsal root ganglion from diabetes to painful neuropathy.

bioRxiv : the preprint server for biology·2026
Same author

Mass-Invariant Natural Log-Transformed Mass Spectra Enable Internal Calibration and <i>De Novo</i> Sequencing of Intact Proteins.

Analytical chemistry·2026

Related Experiment Video

Updated: May 4, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

16.6K

Mass resolution and mass accuracy: how much is enough?

Alan G Marshall1, Greg T Blakney2, Tong Chen3

  • 1Department of Chemistry & Biochemistry, Florida State University ; Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University.

Mass Spectrometry (Tokyo, Japan)
|December 19, 2013
PubMed
Summary

Achieving accurate mass measurement in mass spectrometry requires high resolving power, considering factors beyond peak separation like dynamic range and signal-to-noise ratio. Fourier transform ion cyclotron resonance mass spectrometry offers the highest performance, with recent advancements significantly improving mass accuracy.

Keywords:
FTMSFourier transformion cyclotron resonanceisotopic fine structure

More Related Videos

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Published on: June 10, 2018

24.7K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

13.0K

Related Experiment Videos

Last Updated: May 4, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

16.6K
Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry
06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Published on: June 10, 2018

24.7K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

13.0K

Area of Science:

  • Analytical Chemistry
  • Spectrometry

Background:

  • Accurate mass measurement is crucial for determining elemental composition from mass spectral data.
  • Conventional definitions of mass resolution do not fully account for real-world analytical challenges such as varying peak height ratios and dynamic range.

Purpose of the Study:

  • To redefine the requirements for sufficient mass resolving power in mass spectrometry.
  • To highlight the critical parameters influencing mass accuracy and elemental composition assignment.
  • To discuss advancements in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).

Main Methods:

  • Analysis of mass resolution definitions and their limitations.
  • Identification of key parameters affecting mass resolving power and accuracy: dynamic range, signal-to-noise ratio, digital resolution, and mass-to-charge ratio.
  • Review of recent technological improvements in FT-ICR MS.

Main Results:

  • Mass resolving power requirements are significantly higher (10-100x) than conventionally defined, especially when considering dynamic range and signal-to-noise ratios.
  • Mass accuracy is influenced by signal-to-noise ratio, digital resolution, and isotopic fine structure.
  • FT-ICR MS provides the highest broadband mass resolving power and accuracy, with recent improvements of an order of magnitude.

Conclusions:

  • Sufficient mass resolving power is contingent upon specifying dynamic range, signal-to-noise ratio, digital resolution, and mass-to-charge ratio.
  • Advancements in FT-ICR MS, including higher magnetic fields and improved data processing, have dramatically enhanced mass accuracy.
  • Reliable elemental composition determination necessitates a comprehensive understanding of these influencing factors and the capabilities of high-resolution mass spectrometry techniques.