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Related Experiment Videos

Mast cell secretion: differences between immunologic and non-immunologic stimulation.

B R Rosengard, C Mahalik, D E Cochrane

    Agents and Actions
    |November 1, 1986
    PubMed
    Summary

    Immunologic and non-immunologic mast cell stimulation utilize distinct pathways. Non-immunologic activation relies on intracellular calcium, while immunologic activation uses extracellular calcium, indicating separate signaling mechanisms.

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    Area of Science:

    • Cellular Biology
    • Immunology
    • Biochemistry

    Background:

    • Mast cells play a crucial role in allergic reactions and immune responses.
    • Understanding mast cell activation mechanisms is vital for developing targeted therapies.

    Purpose of the Study:

    • To compare the distinct mechanisms of non-immunologic and immunologic mast cell stimulation.
    • To investigate potential cross-desensitization between these activation pathways.

    Main Methods:

    • Comparative analysis of mast cell responses to non-immunologic (e.g., compound 48/80) and immunologic (antigen-IgE) stimuli.
    • Assessment of calcium (Ca) dependency (intracellular vs. extracellular).
    • Evaluation of neuraminidase treatment effects and 3H-methyl group incorporation into lipids.

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    Main Results:

    • Non-immunologic stimulation primarily uses intracellular Ca, is neuraminidase-insensitive, rapidly inactivates, and does not increase lipid methylation.
    • Immunologic stimulation relies on extracellular Ca, is neuraminidase-sensitive, inactivates slowly, and significantly increases lipid methylation.
    • No cross-inactivation observed between pathways, but neurotensin pretreatment desensitized cells to compound 48/80.

    Conclusions:

    • Immunologic and non-immunologic mast cell activation proceed through separate signaling pathways.
    • Distinct calcium sources and lipid metabolism changes characterize each activation route.
    • Specific pre-treatments can induce pathway-specific desensitization.