Related Experiment Videos
Renal histamine: release by immune stimuli.
Agents and Actions
|November 1, 1986
Summary
Sensitized guinea pig kidneys release histamine upon antigen challenge. Anti-immunoglobulin antibodies also trigger histamine release from both sensitized and non-sensitized kidneys, indicating a role for immune mechanisms in kidney histamine release.
Area of Science:
- Immunology
- Pharmacology
- Renal Physiology
Background:
- Histamine release from immune cells is a key component of allergic reactions.
- The role of histamine and its release mechanisms within kidney tissue is not fully understood.
- Mast cells and basophils are known histamine-releasing cells, but their presence and function in the kidney require further investigation.
Purpose of the Study:
- To investigate the in vitro release of histamine from guinea pig kidneys.
- To determine if specific antigen challenge triggers histamine release in sensitized kidneys.
- To explore the effect of anti-immunoglobulin antibodies on histamine release in both sensitized and non-sensitized kidneys.
Main Methods:
- In vitro perfusion of ovalbumin-sensitized and non-sensitized guinea pig kidneys.
- Challenge with ovalbumin antigen and non-cross-reacting antigens.
- Administration of rabbit antisera to guinea pig IgG1 and IgG2 immunoglobulins.
- Preparation and in vitro study of guinea pig and rat kidney cell suspensions.
- Passive sensitization of rat kidney cells with rat immunoglobulin E (IgE) and challenge with anti-rat IgE antiserum.
- Identification and counting of heparin-containing cells (putative mast cells) using an adapted Technicon H 6000 system.
Main Results:
- Perfused kidneys from ovalbumin-sensitized guinea pigs released significant amounts of histamine upon specific antigen challenge.
- Anti-guinea pig IgG1 and IgG2 antisera induced histamine release from both sensitized and non-sensitized guinea pig kidneys.
- Kidney cell suspensions showed histamine release in response to anti-IgG1 and anti-IgG2 antibodies, and rat kidney cells released histamine upon challenge with anti-rat IgE.
- Heparin-containing cells were rare in kidney tissue, comparable to lung and heart tissues.
Conclusions:
- In vitro perfused kidneys of sensitized guinea pigs release histamine upon specific antigen challenge.
- Both antigen-specific and non-specific (via anti-immunoglobulin antibodies) mechanisms can induce histamine release from kidney cells.
- The presence of mast cells or equivalent cells in the kidney, while rare, may contribute to histamine release.