Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Some histochemical observations on Gaucher cells.

P Bianco, E Bonucci

    Basic and Applied Histochemistry
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    α-catenin switches between a slip and an asymmetric catch bond with F-actin to cooperatively regulate cell junction fluidity.

    Nature communications·2022
    Same author

    Adrenal gland tumors in dairy cattle from Northern Italy: morphological and phenotypical characterization in comparison with human pathology.

    Polish journal of veterinary sciences·2018
    Same author

    An AT-barrier mechanically controls DNA reannealing under tension.

    Nucleic acids research·2016
    Same author

    Spontaneous Age-related Changes of Peripheral Nerves in Cattle: Morphological and Biochemical Studies.

    Anatomia, histologia, embryologia·2015
    Same author

    A technical modification of video-assisted ablation for recurrent pilonidal sinus.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2014
    Same author

    The transition mechanism of DNA overstretching: a microscopic view using molecular dynamics.

    Journal of the Royal Society, Interface·2014

    Concanavalin A (ConA) staining identifies glucocerebrosides in Gaucher cells. This histochemical method, combined with lipid extraction and enzyme digestion, aids in diagnosing Gaucher's disease.

    Area of Science:

    • Biochemistry
    • Histochemistry
    • Cell Biology

    Background:

    • Gaucher's disease is a lysosomal storage disorder.
    • Gaucher cells accumulate glucocerebrosides.
    • Histochemical methods are crucial for disease diagnosis.

    Purpose of the Study:

    • To investigate the binding properties of Concanavalin A (ConA) with Gaucher cells.
    • To explore the potential of ConA staining as a diagnostic tool for Gaucher's disease.
    • To characterize the molecular composition of cytoplasmic inclusions in Gaucher cells.

    Main Methods:

    • Histochemical analysis of plastic-embedded bone marrow biopsies.
    • Concanavalin A (ConA) staining.
    • Lipid extraction and beta-glucosidase digestion assays.

    Related Experiment Videos

  • Electron microscopy.

    • Main Results:

    • ConA binding to cytoplasmic inclusions in Gaucher cells was observed.
    • ConA binding was inhibited by lipid extraction and beta-glucosidase digestion, indicating glucocerebroside involvement.
    • An excess of vic-glycol groups, not lipid-extractable, was detected in Gaucher cells.
    • Electron microscopy revealed regular arrangement of vic-glycols within lysosomes, suggesting lipid-protein interactions.

    Conclusions:

    • Glucocerebrosides stored in Gaucher cells are responsible for ConA binding.
    • ConA staining, coupled with specific biochemical tests, can serve as a diagnostic tool for Gaucher's disease.
    • Lysosomal structures in Gaucher cells exhibit organized lipid-protein interactions, potentially involving acid phosphatase.