Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

17.3K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MAGED1 stabilizes NEUROD1 to promote Per3 expression in the pineal gland.

Life sciences·2026
Same author

Neutrophil extracellular trap (NET)-related index as an indicator of periprosthetic joint infection.

Journal of bone and joint infection·2026
Same author

Electrochemical Oxidation of Water Through Conducting Polymer for Hydroxyl Radical Generation at Ultra-Low Voltage.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Genomic mapping reveals cisplatin disruption of protein phosphorylation signalling genome-wide.

Metallomics : integrated biometal science·2026
Same author

Multi-Dimensional Insights Into the Surface and Interfaces of Battery Materials by Time-of-Flight Secondary Ion Mass Spectrometry.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Molecular unraveling of Li<sup>+</sup>-solvent interaction reversal and its impact on low-temperature desolvation for weakly solvated electrolytes.

Science bulletin·2026
Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026
Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026
Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026
Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026
Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026
Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 4, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K

Fluorescence light-up probe for parallel G-quadruplexes.

Bing Jin1, Xin Zhang, Wei Zheng

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.

Analytical Chemistry
|December 21, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fluorescent probe, BPBC, for selective detection of parallel G-quadruplexes (G4s) in the human genome. This probe offers a sensitive method for studying G4 structures and functions.

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

10.6K

Related Experiment Videos

Last Updated: May 4, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

10.6K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Putative G-quadruplex-forming sequences (PQS) are abundant in the human genome.
  • The structures and functions of most PQSs remain largely uncharacterized.
  • Selective recognition of specific G-quadruplex (G4) types is crucial for their study.

Purpose of the Study:

  • To design and synthesize a novel fluorescent probe for selective G4 detection.
  • To investigate the probe's selectivity towards different G4 structures.
  • To understand the binding mechanism underlying the probe's selectivity.

Main Methods:

  • Design and synthesis of a benzimidazole and carbazole-based fluorescent probe (BPBC).
  • Fluorescence spectroscopy to assess probe response to parallel G4s, antiparallel G4s, and single/double-stranded DNA.
  • Binding studies to elucidate the interaction model between BPBC and G-quartets.

Main Results:

  • BPBC exhibits a crescent-shaped π-conjugated planar core, enabling selective binding to parallel G4s.
  • BPBC shows a significant fluorescence "light-up" effect (330-1800-fold increase) with parallel G4s.
  • Minimal fluorescence enhancement was observed with ss/ds DNA (approx. 30-fold) and antiparallel G4s (30-110-fold).

Conclusions:

  • BPBC is a highly selective fluorescent probe for parallel G-quadruplexes.
  • The probe's selectivity is attributed to an end-stack binding model with G-quartets.
  • BPBC provides a valuable tool for investigating the roles of parallel G4s in the genome.