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Optimization of Radiochemical Reactions using Droplet Arrays
Published on: February 12, 2021
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Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device
Kosuke Ino1, Takehito Goto, Yusuke Kanno
1Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp.
Lab on a Chip
|December 21, 2013
Summary
A novel local redox cycling-based electrochemical (LRC-EC) chip device enables addressable detection of cellular alkaline phosphatase (ALP) activity in single droplets, advancing droplet bioanalysis.
Area of Science:
- Electrochemistry
- Biosensing
- Microfluidics
Background:
- Local redox cycling-based electrochemical (LRC-EC) systems allow for miniaturized sensor arrays.
- Previous LRC-EC systems were limited in droplet array detection capabilities.
Purpose of the Study:
- To fabricate a new LRC-EC chip device for droplet array detection.
- To assess the device's capability for electrochemical detection of redox compounds in droplets.
- To demonstrate the device's application in analyzing cellular bioactivity in droplets.
Main Methods:
- Fabrication of a new LRC-EC chip device incorporating individual Pt pseudo-reference/counter electrodes.
- Utilizing cyclic voltammetry to evaluate the electrochemical performance of the sensor points.
- Applying the device for the detection of alkaline phosphatase (ALP) activity in HeLa cell droplets.
Main Results:
- The fabricated LRC-EC chip device exhibited well-defined voltammograms due to redox cycling at individual sensor points.
- Internal Pt pseudo-reference electrodes facilitated accurate electrochemical measurements in droplets.
- The device successfully enabled addressable detection of ALP activity from HeLa cells in single droplets.
Conclusions:
- The developed LRC-EC chip device is effective for the bioanalysis of droplet systems.
- This technology offers a promising platform for high-throughput droplet-based assays.
- The integration of pseudo-reference electrodes enhances the utility of LRC-EC chips for droplet analysis.
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