Real time hybridization studies by resonant waveguide gratings using nanopattern imaging for Single Nucleotide...

Area of Science:

• Biophysics
• Nanotechnology
• Biosensing

Background:

• Label-free detection using resonant properties of biochips is crucial for multiplexed biosensing.
• Changes in refractive index at the chip surface enable label-free detection.

Purpose of the Study:

• To demonstrate a novel Scanning Of Resonance on Chip by Imaging (SORCI) principle for real-time biological studies.
• To enable multiplexed biosensing through nanopatterned resonant waveguide gratings (RWGs) in a bioarray format.

Main Methods:

• Utilized spatial profiles of nanopatterned RWGs for multiplexed resonance detection.
• Integrated RWGs into a fluidic chip with a black anodized aluminum chamber for enhanced stability and low optical background.
• Performed real-time DNA hybridization experiments for Single Nucleotide Polymorphism (SNP) detection.

Main Results:

• Demonstrated multiplexing of resonance by creating nanopatterned sensing areas in a bioarray.
• Achieved high specificity in detecting DNA targets with close affinity constants.
• Obtained a sensitivity of approximately 10 pg/mm² in buffer solution for real-time biosensing.

Conclusions:

• Validated the applicability of RWGs combined with simple instrumentation for sensitive, real-time biosensing.
• Opened new avenues for various biological studies, including antibiotic resistance evolution.
• Showcased the potential of SORCI for advanced bioanalysis.